使用新型商业检测方法GLABRATA RTT快速鉴定光滑念珠菌
Rapid identification of Candida glabrata with a new commercial test, GLABRATA RTT.
作者信息
Freydiere A-M, Robert R, Ploton C, Marot-Leblond A, Monerau F, Vandenesch F
机构信息
Laboratoire de Bacteriologie, Hôpital Debrousse, Lyon, France.
出版信息
J Clin Microbiol. 2003 Aug;41(8):3861-3. doi: 10.1128/JCM.41.8.3861-3863.2003.
Abstract
The GLABRATA RTT test (Fumouze Diagnostics, Levallois Perret, France) is based on the ability of Candida glabrata to hydrolyze trehalose but not maltose. It requires an inoculum of only four to six colonies, and the results are available within 20 min. We tested GLABRATA RTT with 330 stock isolates grown in subcultures on four different primary fungal isolation media and obtained a sensitivity of 94 to 98% (depending on the medium used) and a specificity of 97.3 to 98.6%. The false-positive results corresponded to C. tropicalis, C. famata, and C. lusitaniae. GLABRATA RTT thus offers rapid and reliable identification of C. glabrata.
摘要
光滑念珠菌RTT试验（法国勒瓦卢瓦-佩雷市的Fumouze诊断公司）基于光滑念珠菌水解海藻糖而非麦芽糖的能力。该试验仅需接种四到六个菌落，20分钟内即可得出结果。我们用在四种不同的主要真菌分离培养基上继代培养的330株保藏菌株对光滑念珠菌RTT试验进行了测试，得到的敏感性为94%至98%（取决于所用培养基），特异性为97.3%至98.6%。假阳性结果对应热带念珠菌、法塔念珠菌和葡萄牙念珠菌。因此，光滑念珠菌RTT试验能快速、可靠地鉴定光滑念珠菌。
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