Department of Laboratory Medicine and Pathology, University of Minnesota, Minnesota, USA.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Acta Biochim Pol. 2021 Mar 7;68(1):77-81. doi: 10.18388/abp.2020_5409.
The global prevalence of type-two diabetes mellitus (T2DM) makes it a disease of public health concern. T2DM is strongly linked with insulin resistance caused by increased levels of visceral fat. Visceral fat secretes several adipocytokines that regulate body metabolism. Resistin is one of these adipocytokines which is encoded by the RETN gene. Herein, we tested the association of the RETN +299(G>A) and -420(C>G) single nucleotide polymorphisms (SNPs) with T2DM. T2DM patients (n=282) and healthy subjects (n=125) were included in the study. Subjects with metabolic syndromes other than diabetes were excluded. Genotyping of subjects was performed using PCR-RFLP. The +299(G>A) SNP was associated with T2DM (P=0.038). The AA genotype was higher in T2DM (17%) compared to controls (8%) with an odd ratio of 2.16 and 95% CI of 1.34 to 4.56. With respect to -420(C>G) SNP, no significant association was found with the risk of T2DM (P=0.128). The haplotype analysis of the examined SNPs indicated that the CA haplotype of the -420 and +299 SNPs in RETN was associated with T2DM risk (P=0.004; odd ration 4.0, 95% CI: 1.56-10.0). In conclusion, the present findings suggest a role of the RETN locus in modulating the risk of T2DM.
全球 2 型糖尿病（T2DM）的患病率使其成为公共卫生关注的疾病。T2DM 与内脏脂肪增加引起的胰岛素抵抗密切相关。内脏脂肪分泌几种调节身体代谢的脂肪细胞因子。抵抗素是其中一种脂肪细胞因子，由 RETN 基因编码。在此，我们检测了 RETN +299(G>A)和-420(C>G)单核苷酸多态性（SNP）与 T2DM 的关联。该研究纳入了 282 名 T2DM 患者和 125 名健康对照者。排除了除糖尿病以外的代谢综合征患者。采用 PCR-RFLP 法对受试者进行基因分型。+299(G>A)SNP 与 T2DM 相关（P=0.038）。AA 基因型在 T2DM 患者中（17%）高于对照组（8%），比值比为 2.16，95%CI 为 1.34 至 4.56。关于-420(C>G)SNP，与 T2DM 风险无显著相关性（P=0.128）。对所研究 SNP 的单体型分析表明，RETN 中-420 和 +299 SNP 的 CA 单体型与 T2DM 风险相关（P=0.004；比值比为 4.0，95%CI：1.56-10.0）。总之，本研究结果提示 RETN 基因座在调节 T2DM 风险中起作用。
