根尖肉芽肿和根端囊肿中ADAMTS-1、多功能蛋白聚糖和磷酸化表皮生长因子受体表达的免疫组织化学分析
Immunohistochemical analysis of ADAMTS-1, versican and pEGFR expressions in periapical granuloma and radicular cyst.
作者信息
Batista Nádia Marielly Gomes, de Moraes Antonia Taiane Lopes, Balbinot Karolyny Martins, de Souza Neto Osvaldo Rodrigues, da Silva Brandão Juliana Melo, da Silva Kataoka Maria Sueli, de Melo Alves Júnior Sérgio, de Jesus Viana Pinheiro João
机构信息
Federal University of Pará Brazil, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil.
Department of Oral Pathology, School of Dentistry, Federal University of Pará, Avenue Augusto Corrêa, 01, Belém, PA, 66075-110, Brazil.
出版信息
BMC Oral Health. 2021 Mar 6;21(1):102. doi: 10.1186/s12903-021-01462-x.
BACKGROUND
ADAMTS expression can be associated with several inflammatory processes, and has been correlated with tumorigenesis of some neoplasms, but its participation in the development of periapical lesions has not been investigated. Therefore, our objective was to verify the expression of ADAMTS-1, versican and pEGFR in Periapical Granuloma (PG) and in the Radicular Cyst (RC) since they are the most common lesions of the periapex.
METHODS
25 samples of RC and 10 of PG were used. As a control, 10 samples of inflammatory fibrous hyperplasia (IFH) and 10 of dental follicle (DF) were used. The expression of these proteins was investigated using immunohistochemistry.
RESULTS
In the epithelium of RC, IFH and DF, the expression of ADAMTS-1 was greater in DF than in RC (p < .001). Versicano showed greater expression in IFH than in RC, DF than in RC (p < .001). pEGFR showed greater expression in IFH and RC than in DF (p < .01 and p < .05, respectively). In connective tissue, ADAMTS-1 expression was greater in PG and RC than in IFH and DF (p < .001). Versicano showed greater expression in PG, RC and IFH compared to DF (p < .001). In pEGFR there was a higher expression in PG when compared to RC, IFH and DF (p < .001). Greater immunostaining occurred in the RC than in the DF (p < .001).
CONCLUSIONS
Our results suggest that the studied proteins may participate in the pathogenesis of PG and RC, through the interaction of these proteins, in the remodeling of the ECM (versican) by ADAMTS-1, producing bioactive fragments, which could activate EGFR, contributing to the formation, growth and maintenance of injuries.
背景
含血小板凝血酶敏感蛋白基元的解聚素样金属蛋白酶（ADAMTS）的表达可能与多种炎症过程相关，并且与某些肿瘤的肿瘤发生有关，但其在根尖周病变发展中的作用尚未得到研究。因此，我们的目的是验证ADAMTS-1、多功能蛋白聚糖和磷酸化表皮生长因子受体（pEGFR）在根尖周肉芽肿（PG）和根端囊肿（RC）中的表达，因为它们是根尖周最常见的病变。
方法
使用了25例RC样本和10例PG样本。作为对照，使用了10例炎症性纤维增生（IFH）样本和10例牙囊（DF）样本。使用免疫组织化学研究这些蛋白的表达。
结果
在RC、IFH和DF的上皮中，ADAMTS-1在DF中的表达高于RC（p＜0.001）。多功能蛋白聚糖在IFH中的表达高于RC，在DF中的表达高于RC（p＜0.001）。pEGFR在IFH和RC中的表达高于DF（分别为p＜0.01和p＜0.05）。在结缔组织中，ADAMTS-1在PG和RC中的表达高于IFH和DF（p＜0.001）。多功能蛋白聚糖在PG、RC和IFH中的表达高于DF（p＜0.001）。与RC、IFH和DF相比，pEGFR在PG中的表达更高（p＜0.001）。RC中的免疫染色比DF中的更强（p＜0.001）。
结论
我们的结果表明，所研究的蛋白可能通过这些蛋白的相互作用，参与PG和RC的发病机制，即ADAMTS-1对细胞外基质（多功能蛋白聚糖）进行重塑，产生生物活性片段，从而激活表皮生长因子受体（EGFR），促进损伤的形成、生长和维持。
Zotero 插件