牙源性角化囊肿中ADAMTS-1及其底物的基因表达和免疫组织化学分析

Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst.

作者信息

de Souza Neto Osvaldo Rodrigues, de Moraes Antonia Taiane Lopes, Fuzii Hellen Thais, Maneschy Faria Antonio Guilherme, Freitas Vanessa Morais, da Silva Kataoka Maria Sueli, de Melo Alves Sérgio, de Jesus Viana Pinheiro João

机构信息

Cell Culture Laboratory, School of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.

Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, 13414-903, Brazil.

出版信息

Diagn Pathol. 2025 Jan 7;20(1):1. doi: 10.1186/s13000-024-01576-0.

DOI:10.1186/s13000-024-01576-0

PMID:39762885

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11705738/

Abstract

BACKGROUND

Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this locally invasive odontogenic cyst.

METHODS

Immunohistochemistry and polymerase chain reaction (PCR) were conducted on 30 cases of odontogenic keratocysts (OKCs) and 20 dental follicles (DFs).

RESULTS

The immunohistochemical expression of these proteins was predominantly cytoplasmic and granular across all samples. In epithelial tissue, the immunoexpression of aggrecan and versican was higher in OKC (p < 0.05) compared to DF. Comparing the expression of proteins between the OKC epithelium and the cystic capsule, it was observed that all molecules were more expressed in the epithelium (p < 0.001). RT-PCR confirmed the expression of ADAMTS-1 and proteoglycans in all samples.

CONCLUSION

ADAMTS-1, aggrecan, brevican, and versican were expressed in all samples with a granular and cytoplasmic pattern. RT-PCR confirmed their presence in both OKC and DF, but only aggrecan and versican exhibited significantly higher levels in OKC (p < 0.05). Protein expression was notably greater in the epithelial component of OKC. These findings underscore the potential role of these proteins in the biological behavior of OKC.

摘要

背景

鉴于微环境在牙源性角化囊肿局部侵袭性中起重要作用，本研究旨在评估含金属蛋白酶与凝血酶敏感蛋白基序的蛋白1（ADAMTS-1）及其底物多功能蛋白聚糖、聚集蛋白聚糖和短蛋白聚糖在这种局部侵袭性牙源性囊肿中的表达。

方法

对30例牙源性角化囊肿（OKC）和20个牙囊（DF）进行免疫组织化学和聚合酶链反应（PCR）检测。

结果

所有样本中这些蛋白的免疫组织化学表达主要位于细胞质且呈颗粒状。在上皮组织中，与DF相比，OKC中聚集蛋白聚糖和多功能蛋白聚糖的免疫表达更高（p < 0.05）。比较OKC上皮和囊壁中蛋白的表达，发现所有分子在上皮中的表达更高（p < 0.001）。逆转录-聚合酶链反应（RT-PCR）证实所有样本中均有ADAMTS-1和蛋白聚糖表达。

结论

ADAMTS-1、聚集蛋白聚糖、短蛋白聚糖和多功能蛋白聚糖在所有样本中均呈颗粒状和细胞质模式表达。RT-PCR证实它们在OKC和DF中均存在，但只有聚集蛋白聚糖和多功能蛋白聚糖在OKC中的水平显著更高（p < 0.05）。OKC的上皮成分中蛋白表达明显更高。这些发现强调了这些蛋白在OKC生物学行为中的潜在作用。