五个小麦-中间偃麦草部分双二倍体的分子细胞遗传学特征及对赤霉病的抗性

Molecular cytogenetic characterization and fusarium head blight resistance of five wheat-Thinopyrum intermedium partial amphiploids.

作者信息

Wang Hui, Cheng Shuwei, Shi Yue, Zhang Shuxin, Yan Wei, Song Weifu, Yang Xuefeng, Song Qingjie, Jang Bo, Qi Xiaoyue, Li Xinling, Friebe Bernd, Zhang Yanming

机构信息

Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.

Crop Resources Institute, Heilongjiang Academy of Agriculture Sciences, Harbin, 150086, China.

出版信息

Mol Cytogenet. 2021 Mar 6;14(1):15. doi: 10.1186/s13039-021-00536-3.

DOI:10.1186/s13039-021-00536-3

PMID:33676531

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7937273/

Abstract

BACKGROUND

Partial amphiploids created by crossing octoploid tritelytrigia(2n = 8× = 56, AABBDDEE) and Thinopyrum intermedium (2n = 6× = 42, StStJJJJ) are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat-Th. intermedium partial amphiploids using GISH and multicolor-FISH.

RESULTS

The result revealed that five lines had 10-14 J-genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J-genomic DNA from Th. bessarabicum as GISH probe and the oligo probes pAs1-1, pAs1-3, AFA-4, (GAA) 10, and pSc119.2-1 as FISH probe. Five lines resembled their parent octoploid tritelytrigia (2n = 8× = 56, AABBDDEE) but had higher protein contents. Protein contents of two lines HS2-2 and HS2-5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2-2, HS2-4, HS2-5, and HS2-16 were less than 20% of PPS. Line HS2-5 with 14 J-genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively.

CONCLUSIONS

New wheat-Th. intermedium amphiploids with the J-genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.

摘要

背景

通过八倍体小偃麦（2n = 8× = 56，AABBDDEE）与中间偃麦草（2n = 6× = 42，StStJJJJ）杂交产生的部分双二倍体，因其对小麦主要病害具有抗性，是小麦育种中的重要中间材料。我们利用基因组原位杂交（GISH）和多色荧光原位杂交（FISH）技术检测了5个小麦-中间偃麦草部分双二倍体的染色体组成。

结果

以贝加尔湖偃麦草的J基因组DNA作为GISH探针，寡核苷酸探针pAs1-1、pAs1-3、AFA-4、(GAA)10和pSc119.2-1作为FISH探针，结果显示，5个株系含有来自中间偃麦草的10 - 14条J基因组染色体和42条普通小麦染色体。5个株系与它们的亲本八倍体小偃麦（2n = 8× = 56，AABBDDEE）相似，但蛋白质含量更高。其中HS2-2和HS2-5两个株系的蛋白质含量高达20%以上。对赤霉病抗性的评估显示，这些株系的病小穗率（PSS）均低于30%。HS2-2、HS2-4、HS2-5和HS2-16株系的PPS小于20%。含有14条来自中间偃麦草J基因组染色体的HS2-5株系表现出最佳的抗病性，2016年和2017年的PSS值分别为10.8%和16.6%。