A diencephalic slice preparation and chamber for studying neuronal thermosensitivity.

An in vitro slice preparation and electrophysiological recording chamber for studying neuronal thermosensitivity throughout the hypothalamus are described. A series of eight, 300 microns thick, horizontal tissue slices encompassing most of the hypothalamus are prepared from male Sprague-Dawley rats. Horizontal slice maps showing the major nuclei and fiber tracts are provided. Horizontal tissue slices contain many hypothalamic nuclei as well as the medial forebrain bundle, a large fiber tract interconnecting these nuclei. Three water-perfused thermodes directly beneath the tissue slices are used to produce discrete thermal stimulations of rostral, middle, and caudal nuclear regions. Fine thermocouples monitor slice temperature over each thermode. Limiting microelectrode explorations to regions directly over a thermode eliminates the problems of temperature gradients and permits more accurate manipulation of temperature at the recording site. While this preparation is ideal for characterizing hypothalamic neuronal thermosensitivity, it is also appropriate for electrophysiological studies of other hypothalamic functional systems.