Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, Indiana 46556, USA.
Department of Computer Science and Engineering, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, Indiana 46556, USA.
Trends Biotechnol. 2022 Jan;40(1):22-37. doi: 10.1016/j.tibtech.2021.02.008. Epub 2021 Mar 3.
The drastically increasing amount of plastic waste is causing an environmental crisis that requires innovative technologies for recycling post-consumer plastics to achieve waste valorization while meeting environmental quality goals. Biocatalytic depolymerization mediated by enzymes has emerged as an efficient and sustainable alternative for plastic treatment and recycling. A variety of plastic-degrading enzymes have been discovered from microbial sources. Meanwhile, protein engineering has been exploited to modify and optimize plastic-degrading enzymes. This review highlights the recent trends and up-to-date advances in mining novel plastic-degrading enzymes through state-of-the-art omics-based techniques and improving the enzyme catalytic efficiency and stability via various protein engineering strategies. Future research prospects and challenges are also discussed.
急剧增加的塑料废物量正在造成一场环境危机，这需要创新的技术来回收消费后的塑料，以实现废物增值，同时达到环境质量目标。酶介导的生物催化解聚已成为处理和回收塑料的一种有效和可持续的替代方法。已经从微生物来源中发现了多种塑料降解酶。同时，蛋白质工程也被用于修饰和优化塑料降解酶。本文综述了通过最新的基于组学的技术来挖掘新型塑料降解酶的最新趋势和最新进展，以及通过各种蛋白质工程策略来提高酶的催化效率和稳定性。还讨论了未来的研究前景和挑战。
