Li Jiang, Wang Yi, Wei Hairong, Kang Xiangyang
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, PR China.
Zhongkai University of Agriculture and Engineering, Guangzhou 510225, PR China.
Genomics. 2021 May;113(3):1180-1192. doi: 10.1016/j.ygeno.2021.03.002. Epub 2021 Mar 5.
Though allotriploid poplar shows a salient vegetative growth advantage that impacts biomass and lumber yield, the proteomic data of Populus allotriploids have not been scrutinized for identifying the underlying molecular mechanisms. We conducted a large-scale label-free proteomics profiling of the 5th, 10th, and 25th leaves of allotriploids and diploids, and identified 4587 protein groups. Among 932 differentially expressed proteins (DEPs), 22 are transcription factors (TFs) that could regulate vegetative growth advantage in allotriploids. The DEPs involved in light reaction, Calvin cycle, and photorespiration, protein synthesis, sucrose synthesis, starch synthesis, and starch decomposition displayed elevated expression in Populus allotriploids. However, the DEPs functioning in sucrose decomposition, tricarboxylic acid (TCA) cycle, and protein degradation exhibited significantly downregulated expression. The alternations of these DEPs augmented efficiency of photosynthesis, carbon fixation, sucrose and starch accumulation, and decreased capacity of carbohydrate consumption, leading to larger volume of biomass and vigorous growth in Populus allotriploids.
尽管异源三倍体杨树表现出显著的营养生长优势，这对生物量和木材产量有影响，但尚未对杨树异源三倍体的蛋白质组数据进行仔细研究以确定其潜在的分子机制。我们对异源三倍体和二倍体的第5片、第10片和第25片叶子进行了大规模的无标记蛋白质组分析，共鉴定出4587个蛋白质组。在932个差异表达蛋白（DEP）中，有22个是转录因子（TF），它们可能调控异源三倍体的营养生长优势。参与光反应、卡尔文循环、光呼吸、蛋白质合成、蔗糖合成、淀粉合成和淀粉分解的DEP在杨树异源三倍体中表达上调。然而，在蔗糖分解、三羧酸（TCA）循环和蛋白质降解中起作用的DEP表达显著下调。这些DEP的变化提高了光合作用、碳固定、蔗糖和淀粉积累的效率，并降低了碳水化合物消耗的能力，导致杨树异源三倍体生物量更大、生长更旺盛。
