《Mitragyna speciosa（Kratom）基因组：挖掘影响人类健康的有效药物的资源》。
The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health.
机构信息
Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.
Department of Chemistry, University of British Columbia Okanagan, Kelowna, BC V1V 1V7, Canada.
出版信息
G3 (Bethesda). 2021 Apr 15;11(4). doi: 10.1093/g3journal/jkab058.
Mitragyna speciosa (kratom) produces numerous compounds with pharmaceutical properties including the production of bioactive monoterpene indole and oxindole alkaloids. Using a linked-read approach, a 1,122,519,462 bp draft assembly of M. speciosa "Rifat" was generated with an N50 scaffold size of 1,020,971 bp and an N50 contig size of 70,448 bp that encodes 55,746 genes. Chromosome counting revealed that "Rifat" is a tetraploid with a base chromosome number of 11, which was further corroborated by orthology and syntenic analysis of the genome. Analysis of genes and clusters involved in specialized metabolism revealed genes putatively involved in alkaloid biosynthesis. Access to the genome of M. speciosa will facilitate an improved understanding of alkaloid biosynthesis and accelerate the production of bioactive alkaloids in heterologous hosts.
异叶清风藤（Kratom）产生了许多具有药物特性的化合物，包括生物活性单萜吲哚和氧化吲哚生物碱的产生。使用链接读取方法，生成了 M. speciosa "Rifat" 的 1,122,519,462bp 草图组装，N50 支架大小为 1,020,971bp，N50 连续体大小为 70,448bp，编码 55,746 个基因。染色体计数表明，"Rifat"是一个四倍体，基数染色体数为 11，这进一步通过基因组的同源性和共线性分析得到证实。对参与特化代谢的基因和基因簇的分析揭示了可能参与生物碱生物合成的基因。获得 M. speciosa 的基因组将有助于更好地理解生物碱生物合成，并加速在异源宿主中产生生物活性生物碱。
Zotero 插件