Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
J Agric Food Chem. 2021 Mar 17;69(10):3032-3043. doi: 10.1021/acs.jafc.0c04575. Epub 2021 Mar 6.
Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype. In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry. 16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants. The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while genus were highly enriched in infants fed by non-secretor mothers. Associations between HMOs and infant fecal microbiota showed that the relative abundance of -OTU158 was positively associated with 2'-fucosyllactose and 3-fucosyllactose, and -OTU90 was negatively associated with lacto--difucohexaose. The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different strains in species-specific manner.
人乳寡糖 (HMOs) 在塑造婴儿粪便微生物群方面发挥着关键作用，并且据报道 HMOs 谱因母亲的糖基转移酶表型而异。在这项研究中，通过液相色谱四极杆飞行时间串联质谱法分析了产后 2 个月时分泌型或非分泌型母亲的母乳中 HMOs 的谱。16S rRNA 测序用于表征母乳喂养婴儿的粪便微生物群。分泌型母亲的总 HMO 和岩藻糖基化 HMO 的含量高于非分泌型母亲，而非分泌型母亲喂养的婴儿中 属高度富集。HMO 与婴儿粪便微生物群之间的关联表明，-OTU158 的相对丰度与 2'-岩藻糖基乳糖和 3-岩藻糖基乳糖呈正相关，-OTU90 与乳-二岩藻六糖呈负相关。本研究提供了中国母亲的 HMO 谱及其与婴儿粪便微生物群组成的关联，表明 HMO 组成与特定物种中的不同 菌株相关。
