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多组学分析以检测来自多部位脂肪间充质干细胞的基因表达和代谢产物

Multi-Omics Analysis to Examine Gene Expression and Metabolites From Multisite Adipose-Derived Mesenchymal Stem Cells.

作者信息

Yang Chuanxi, Zhang Jing, Wu Tingting, Zhao Kun, Wu Xiaoguang, Shi Jing, Sun Wei, Kong Xiangqing

机构信息

Department of Cardiology, Medical School of Southeast University, Nanjing, China.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

出版信息

Front Genet. 2021 Feb 18;12:627347. doi: 10.3389/fgene.2021.627347. eCollection 2021.

DOI:10.3389/fgene.2021.627347
PMID:33679891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7930907/
Abstract

This study aimed at exploring the gene expression and metabolites among multisite adipose-derived mesenchymal stem cells (ASCs) and investigate the metabolic pathway using a multi-omics analysis. Subcutaneous adipose-derived mesenchymal stem cells (SASCs), perirenal adipose-derived mesenchymal stem cells (PASCs), and epididymal adipose-derived mesenchymal stem cells (EASCs) were isolated from Sprague Dawley rats. RNA and metabolites were extracted and sequenced using transcriptomics and metabolomics analyses, respectively. There were 720 differentially expressed genes (DEGs) in EASCs and 688 DEGs in PASCs compared with SASCs; there were 166 unique DEGs in EASCs, 134 unique DEGs in PASCs, and 554 common DEGs between EASCs and PASCs. Furthermore, there were 226 differential metabolites in EASCs, 255 differential metabolites in PASCs, 83 unique differential metabolites in EASCs, 112 unique differential metabolites in PASCs, and 143 common differential metabolites between EASCs and PASCs. The transcriptomics and metabolomics analyses identified four hub genes, one in EASCs and three in PASCs. There are functional differences among multisite ASCs that may be related to the hub genes , , , and . The relevant signaling pathways are the Ras signaling pathway, HIF-1 signaling pathway, and the p53 signaling pathway. In conclusion, compared with SASCs, our multi-omics analysis identified that EASCs with higher expression may be more correlated to fat metabolism and insulin resistance, while PASCs with abnormal expression of and may be more correlated with some malignant tumors and cardiac-cerebral vascular disease.

摘要

本研究旨在探索多部位脂肪来源间充质干细胞（ASC）中的基因表达和代谢物，并使用多组学分析研究代谢途径。从Sprague Dawley大鼠中分离出皮下脂肪来源间充质干细胞（SASC）、肾周脂肪来源间充质干细胞（PASC）和附睾脂肪来源间充质干细胞（EASC）。分别使用转录组学和代谢组学分析提取RNA和代谢物并进行测序。与SASC相比，EASC中有720个差异表达基因（DEG），PASC中有688个DEG；EASC中有166个独特的DEG，PASC中有134个独特的DEG，EASC和PASC之间有554个共同的DEG。此外，EASC中有226种差异代谢物，PASC中有255种差异代谢物，EASC中有83种独特的差异代谢物，PASC中有112种独特的差异代谢物，EASC和PASC之间有143种共同的差异代谢物。转录组学和代谢组学分析确定了四个枢纽基因，一个在EASC中，三个在PASC中。多部位ASC之间存在功能差异，可能与枢纽基因 、 、 和 有关。相关信号通路为Ras信号通路、HIF-1信号通路和p53信号通路。总之，与SASC相比，我们的多组学分析表明， 表达较高的EASC可能与脂肪代谢和胰岛素抵抗更相关，而 和 表达异常的PASC可能与一些恶性肿瘤和心脑血管疾病更相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/224a/7930907/2c8b1ec7c582/fgene-12-627347-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/224a/7930907/092aaf1ef31d/fgene-12-627347-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/224a/7930907/2c8b1ec7c582/fgene-12-627347-g009.jpg
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