Technol Health Care. 2021;29(S1):531-538. doi: 10.3233/THC-218050.
We aimed to investigate the effects of hydroxyapatite bioceramic extract on Ang/Tie2 system and cell proliferation of umbilical vein endothelial cells.
Human umbilical vein endothelial cells (HUVECs) were used in this research. There are two induvial groups, control group and hydroxyapatite bioceramics extract treatment group. Cell Counting Kit-8 (CCK-8) was used to evaluate cell proliferation. Western blot and real time quantitative PCR (Q-PCR) were used to evaluate the protein and mRNA expression levels of Ang1, Ang2 and Tie2 in Ang/Tie2 system, respectively. All the results were statistically analyzed by Spss19.0. All data were presented as mean ± standard error of mean (SEM). Student's t-test was performed to determine the differences among grouped data.
Hydroxyapatite bioceramics extract showed no effect on the cell morphology and cell proliferation of HUVECs. Interestingly, we found that both Ang2 and Tie2 protein and mRNA level were markedly increased by hydroxyapatite bioceramics extract.
Hydroxyapatite bioceramic extract showed no cytotoxicity to HUVECs, and might regulate vascular remodeling by mediating Ang/Tie2 system.
研究羟基磷灰石生物陶瓷提取物对血管生成素（Ang）/血管生成素受体 2（Tie2）系统和脐静脉内皮细胞增殖的影响。
本研究采用人脐静脉内皮细胞（HUVEC）。分为对照组和羟基磷灰石生物陶瓷提取物处理组。使用细胞计数试剂盒-8（CCK-8）评估细胞增殖。Western blot 和实时定量 PCR（Q-PCR）分别用于评估 Ang/Tie2 系统中 Ang1、Ang2 和 Tie2 的蛋白和 mRNA 表达水平。所有结果均采用 Spss19.0 进行统计学分析。所有数据均表示为均数±标准误（SEM）。采用 Student's t 检验比较组间数据的差异。
羟基磷灰石生物陶瓷提取物对 HUVECs 的细胞形态和细胞增殖没有影响。有趣的是，我们发现羟基磷灰石生物陶瓷提取物可显著增加 Ang2 和 Tie2 的蛋白和 mRNA 水平。
羟基磷灰石生物陶瓷提取物对 HUVECs 无细胞毒性，可能通过调节 Ang/Tie2 系统来调节血管重塑。
