Center of Excellence Food Technology and Nutrition, University of Applied Sciences Upper Austria, Wels, Austria.
FFoQSI GmbH-Austrian Competence Centre for Feed and Food Quality, Safety and Innovation, Tulln, Austria.
Crit Rev Food Sci Nutr. 2022;62(22):5965-5982. doi: 10.1080/10408398.2021.1895060. Epub 2021 Mar 8.
In the field of (food) toxicology, there is a strong trend of replacing animal trials with alternative methods for the assessment of adverse health effects in humans. The replacement of animal trials is not only driven by ethical concerns but also by the number of potential testing substances (food additives, packaging material, contaminants, and toxicants), which is steadily increasing. In vitro 2D cell culture applications in combination with modeling might provide an applicable first response. However, those systems lack accurate predictions of metabolic actions. Thus, alternative in vivo models could fill the gap between cell culture and animal trials. In this review, we highlight relevant studies in the field and spotlight the applicability of alternative models, including , , , HET-CAM and Lab-on-a-chip.
在（食品）毒理学领域，人们强烈倾向于用替代方法取代动物试验，以评估对人类的不良健康影响。动物试验的替代不仅是出于伦理方面的考虑，也是因为潜在的测试物质（食品添加剂、包装材料、污染物和毒物）的数量在稳步增加。体外 2D 细胞培养应用与建模相结合可能提供一种可行的初步反应。然而，这些系统缺乏对代谢作用的准确预测。因此，替代的体内模型可以填补细胞培养和动物试验之间的空白。在这篇综述中，我们强调了该领域的相关研究，并突出了替代模型的适用性，包括鱼、昆虫幼虫、斑马鱼、鸡胚绒毛尿囊膜和芯片实验室。
