Rumata Nur R, Purwaningsih Dewi, Asbah Asbah, As'ad Muh Fadhil, Chadran Deepak, Emran Talha B, Nainu Firzan
Sekolah Tinggi Ilmu Farmasi Makassar, Makassar, Indonesia.
Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia.
Narra J. 2023 Aug;3(2):e117. doi: 10.52225/narra.v3i2.117. Epub 2023 Jun 11.
Curcumin, a dietary polyphenol derived from turmeric's rhizome, exhibits a range of pharmacological activities, such as antioxidant, analgesic, antipyretic, and anti-inflammatory effects. It has been investigated for its therapeutic potential in chronic obstructive pulmonary disease (COPD) due to its ability to inhibit nuclear factor kappa B (NF-κB), modulate oxidative stress, impact cell viability, and regulate gene expression. However, most studies have been limited to in vitro conditions. To address this gap, we conducted in vivo experiments using the fruit fly to explore the antioxidant activities and biological significance of curcumin. Several parameters were assessed using different assays, including curcumin toxicity assay, fly survival, locomotor response to curcumin treatment (with or without cigarette smoke), trypan blue staining, larval crawling assays, and gene expression analysis. Our data revealed no significant differences in toxicity and locomotor tests across various curcumin concentrations. tolerated curcumin at concentrations of 0.5 μM, 5 μM, 50 μM, and 500 μM, suggesting its safety without negatively impacting locomotor. Furthermore, curcumin at 5 μM extended the lifespan of exposed to cigarette smoke, while reversing the negative effects of smoke exposure on gut cell viability and larval locomotor activity. In conclusion, curcumin administration appeared safe for , with potential benefits for longevity and locomotory function. These findings support the idea that curcumin possesses in vivo antioxidant properties and may serve as a promising pharmacological agent. However, further study is needed to explore its potential applications in human health and disease management, particularly in the context of COPD.
姜黄素是一种从姜黄根茎中提取的膳食多酚，具有一系列药理活性，如抗氧化、镇痛、解热和抗炎作用。由于其能够抑制核因子κB（NF-κB）、调节氧化应激、影响细胞活力和调节基因表达，人们对其在慢性阻塞性肺疾病（COPD）中的治疗潜力进行了研究。然而，大多数研究仅限于体外条件。为了填补这一空白，我们使用果蝇进行了体内实验，以探索姜黄素的抗氧化活性和生物学意义。使用不同的测定方法评估了几个参数，包括姜黄素毒性测定、果蝇存活率、对姜黄素处理（有无香烟烟雾）的运动反应、台盼蓝染色、幼虫爬行测定和基因表达分析。我们的数据显示，在各种姜黄素浓度下，毒性和运动测试没有显著差异。果蝇在0.5μM、5μM、50μM和500μM浓度下耐受姜黄素，表明其安全性，且不会对运动产生负面影响。此外，5μM的姜黄素延长了暴露于香烟烟雾中的果蝇的寿命，同时逆转了烟雾暴露对肠道细胞活力和幼虫运动活性的负面影响。总之，姜黄素给药对果蝇似乎是安全的，对寿命和运动功能有潜在益处。这些发现支持了姜黄素具有体内抗氧化特性的观点，并且可能成为一种有前途的药物。然而，需要进一步研究以探索其在人类健康和疾病管理中的潜在应用，特别是在COPD的背景下。
