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微小 RNA 家族 miR-44 促进秀丽隐杆线虫雌雄同体生殖系精子命运。

Sperm fate is promoted by the mir-44 microRNA family in the Caenorhabditis elegans hermaphrodite germline.

机构信息

Department of Biological Sciences, Marquette University, 1428 W. Clybourn Ave, PO Box 1881, Milwaukee, WI 53233, USA.

出版信息

Genetics. 2021 Mar 3;217(1):1-14. doi: 10.1093/genetics/iyaa006.

Abstract

Posttranscriptional regulation of gene expression, typically effected by RNA-binding proteins, microRNAs (miRNAs), and translation initiation factors, is essential for normal germ cell function. Numerous miRNAs have been detected in the germline; however, the functions of specific miRNAs remain largely unknown. Functions of miRNAs have been difficult to determine as miRNAs often modestly repress target mRNAs and are suggested to sculpt or fine tune gene expression to allow for the robust expression of cell fates. In Caenorhabditis elegans hermaphrodites, cell fate decisions are made for germline sex determination during larval development when sperm are generated in a short window before the switch to oocyte production. Here, analysis of newly generated mir-44 family mutants has identified a family of miRNAs that modulate the germline sex determination pathway in C. elegans. Mutants with the loss of mir-44 and mir-45 produce fewer sperm, showing both a delay in the specification and formation of sperm as well as an early termination of sperm specification accompanied by a premature switch to oocyte production. mir-44 and mir-45 are necessary for the normal period of fog-1 expression in larval development. Through genetic analysis, we find that mir-44 and mir-45 may act upstream of fbf-1 and fem-3 to promote sperm specification. Our research indicates that the mir-44 family promotes sperm cell fate specification during larval development and identifies an additional posttranscriptional regulator of the germline sex determination pathway.

摘要

基因表达的转录后调控,通常由 RNA 结合蛋白、microRNAs(miRNAs)和翻译起始因子来完成,对于正常的生殖细胞功能至关重要。在线虫的生殖系中已经检测到许多 miRNAs,但特定 miRNAs 的功能仍然知之甚少。由于 miRNAs 通常会适度抑制靶 mRNA 的表达,并且被认为可以塑造或微调基因表达,以允许细胞命运的稳健表达,因此 miRNAs 的功能很难确定。在线虫的雌雄同体中,生殖细胞的命运决定是在幼虫发育过程中进行的,此时在向卵母细胞生成转变之前的一个短暂窗口中产生精子。在这里,对新生成的 mir-44 家族突变体的分析确定了一组 miRNA,它们在 C. elegans 中调节生殖细胞性别决定途径。mir-44 和 mir-45 缺失的突变体产生的精子较少,显示出精子的特化和形成延迟,以及精子特化的早期终止,伴随着卵母细胞生成的过早转变。mir-44 和 mir-45 对于幼虫发育过程中 fog-1 表达的正常时期是必要的。通过遗传分析,我们发现 mir-44 和 mir-45 可能在上游作用于 fbf-1 和 fem-3 以促进精子特化。我们的研究表明,mir-44 家族在幼虫发育过程中促进精子细胞命运特化,并确定了生殖细胞性别决定途径的另一个转录后调节剂。

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本文引用的文献

1
Distinct roles of two eIF4E isoforms in the germline of .
J Cell Sci. 2020 Mar 30;133(6):jcs237990. doi: 10.1242/jcs.237990.
2
MirGeneDB 2.0: the metazoan microRNA complement.
Nucleic Acids Res. 2020 Jan 8;48(D1):D132-D141. doi: 10.1093/nar/gkz885.
3
Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.
PLoS Genet. 2019 Feb 8;15(2):e1007905. doi: 10.1371/journal.pgen.1007905. eCollection 2019 Feb.
4
Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development.
Nat Commun. 2018 Dec 14;9(1):5318. doi: 10.1038/s41467-018-07791-w.
5
Toward Identifying Subnetworks from FBF Binding Landscapes in Spermatogenic or Oogenic Germlines.
G3 (Bethesda). 2019 Jan 9;9(1):153-165. doi: 10.1534/g3.118.200300.
6
Spatiotemporal Gene Expression Analysis of the Germline Uncovers a Syncytial Expression Switch.
Genetics. 2018 Oct;210(2):587-605. doi: 10.1534/genetics.118.301315. Epub 2018 Aug 9.
7
Recent Molecular Genetic Explorations of MicroRNAs.
Genetics. 2018 Jul;209(3):651-673. doi: 10.1534/genetics.118.300291.
8
Initiation of Meiotic Development Is Controlled by Three Post-transcriptional Pathways in .
Genetics. 2018 Aug;209(4):1197-1224. doi: 10.1534/genetics.118.300985. Epub 2018 Jun 25.
9
Metazoan MicroRNAs.
Cell. 2018 Mar 22;173(1):20-51. doi: 10.1016/j.cell.2018.03.006.
10
The cluster represses receptor tyrosine kinase signaling to determine cell fates in the eye.
Development. 2018 Apr 9;145(7):dev159053. doi: 10.1242/dev.159053.

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