Department of Public Health, Faculty of Health Sciences, Medical University of Bialystok, Bialystok, Poland.
Department of Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, Bialystok, Poland.
Adv Med Sci. 2021 Mar;66(1):185-191. doi: 10.1016/j.advms.2021.02.004. Epub 2021 Mar 5.
Normal endothelial function is important for the homeostasis of the cardiovascular (CV) system. The aim of the present study was to determine the profile of key parameters of endothelial dysfunction in middle-aged men that play a significant role in the functioning of endothelial vessels, which seems to be crucial for the early diagnosis of cardiovascular disorders.
The study included 53 men, 20 with hypertension (HTN), 18 with HTN and related diseases, 15 healthy controls Apart from general testing (BMI, biochemical analysis, SBP, DBP), we used the Griess reaction to assess the total amount of nitric oxide (NO), and used ELISA to verify the concentrations of malondialdehyde (MDA), nitrotyrosine (NT), asymmetric dimethylarginine (ADMA), tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein 1 (MCP-1), and myeloperoxidase (MPO). Furthermore, we assessed the concentration of circulating free DNA (cfDNA) using the fluorescence method.
The values of MDA, ADMA, cfDNA, and MPO observed in samples from men with HTN were determined to be higher compared to those from men without HTN. In the group of men with HTN and other concomitant cardiovascular disorders, we observed low concentrations of NO, MDA, and ADMA with high concentrations of cfDNA.
The results obtained for parameters selected for the study, should be considered by cardiologists as a prompt to include in the diagnostic profile the assessment of NO and cfDNA concentrations for risk evaluation and/or diagnosis of endothelial dysfunction in patients suffering from HTN or related complications.
正常的内皮功能对于心血管系统的稳态至关重要。本研究旨在确定在中年男性中，与内皮功能障碍相关的关键参数谱，这些参数在血管内皮功能中起着重要作用，对于心血管疾病的早期诊断似乎至关重要。
该研究纳入了 53 名男性，其中 20 名患有高血压（HTN），18 名患有 HTN 及其相关疾病，15 名健康对照者。除了一般检查（BMI、生化分析、SBP、DBP）外，我们还使用格里斯反应评估了一氧化氮（NO）的总量，并使用 ELISA 验证了丙二醛（MDA）、硝基酪氨酸（NT）、非对称二甲基精氨酸（ADMA）、肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白 1（MCP-1）和髓过氧化物酶（MPO）的浓度。此外，我们还使用荧光法评估了循环游离 DNA（cfDNA）的浓度。
与无高血压的男性相比，患有 HTN 的男性样本中的 MDA、ADMA、cfDNA 和 MPO 值被确定为更高。在患有 HTN 和其他并发心血管疾病的男性组中，我们观察到 NO、MDA 和 ADMA 浓度低，cfDNA 浓度高。
对于所选研究参数获得的结果，应被心脏病学家视为提示，将 NO 和 cfDNA 浓度的评估纳入患有 HTN 或相关并发症的患者的诊断谱中，以进行风险评估和/或内皮功能障碍的诊断。
