人血液中类DSIP物质的放射免疫测定：可能的蛋白质结合

Radioimmunoassay of DSIP-like material in human blood: possible protein binding.

作者信息

Kastin A J, Castellanos P F, Banks W A, Coy D H

出版信息

Pharmacol Biochem Behav. 1981 Dec;15(6):969-74. doi: 10.1016/0091-3057(81)90064-2.

Abstract

A radioimmunoassay (RIA) for DSIP-like material was established in unextracted human plasma. Most of the immunoreactivity was found in a "large" fraction while a much smaller amount co-eluted with DSIP from Sephadex as a "free" fraction. Both peaks progressively increased with increasing amounts of added DSIP. Acidification, but not treatment with charcoal-dextran, resulted in a relative decrease in the "large" peak and an increase in the "free" peak. This RIA, therefore, appears to measure both bound and free forms of DSIP-like immunoreactivity, the levels of which were higher at 4 p.m. than at 8 a.m.

摘要

在未经提取的人血浆中建立了一种针对类DSIP物质的放射免疫分析（RIA）方法。大部分免疫反应性存在于“大分子”部分，而与DSIP从葡聚糖凝胶中共同洗脱的作为“游离”部分的量要少得多。随着添加的DSIP量增加，两个峰均逐渐升高。酸化而非用炭-葡聚糖处理导致“大分子”峰相对降低，“游离”峰升高。因此，这种RIA似乎能检测类DSIP免疫反应性的结合形式和游离形式，其水平在下午4点高于上午8点。