Department of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang, People's Republic of China.
Department of Physiology and Neurobiology, Mudanjiang Medical University, Mudanjiang 157000, Heilongjiang, People's Republic of China.
Aging (Albany NY). 2021 Mar 3;13(5):7430-7453. doi: 10.18632/aging.202604.
Glucosamine-phosphate N-acetyltransferase 1 (GNPNAT1) is a key enzyme associated with glucose metabolism and uridine diphosphate-N-acetylglucosamine biosynthesis. Abnormal expression might be associated with carcinogenesis. We analyzed multiple lung adenocarcinoma (LUAD) gene expression databases and verified higher expression in LUAD tumor tissues than in normal tissues. Moreover, we analyzed the survival relationship between LUAD patients' clinical status and expression, and found higher expression in LUAD patients with unfavorable prognosis. We built gene co-expression networks and further annotated the co-expressed genes' Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and various associated regulatory factors. These co-expression genes' functional networks mainly participate in chromosome segregation, RNA metabolic process, and RNA transport. We analyzed genetic alterations and co-occurrence networks, and the functional networks of these genes showed that participates in multiple steps of cell cycle transition and in the development of some cancers. We assessed the correlation between expression and cancer immune infiltrates and showed that expression is correlated with several immune cells, chemokines, and immunomodulators in LUAD. We found that correlates with LUAD development and prognosis, laying a foundation for further research, especially in immunotherapy.
葡萄糖胺-磷酸 N-乙酰转移酶 1（GNPNAT1）是与葡萄糖代谢和尿苷二磷酸-N-乙酰葡萄糖胺生物合成相关的关键酶。异常表达可能与癌变有关。我们分析了多个肺腺癌（LUAD）基因表达数据库，并证实 LUAD 肿瘤组织中的表达高于正常组织。此外，我们分析了 LUAD 患者临床状况与表达之间的生存关系，发现预后不良的 LUAD 患者表达较高。我们构建了基因共表达网络，并进一步注释了共表达基因的基因本体论（GO）术语、京都基因与基因组百科全书（KEGG）途径以及各种相关调节因子。这些共表达基因的功能网络主要参与染色体分离、RNA 代谢过程和 RNA 运输。我们分析了遗传改变和共发生网络，这些基因的功能网络表明参与了细胞周期转换的多个步骤以及某些癌症的发展。我们评估了 表达与癌症免疫浸润之间的相关性，并表明在 LUAD 中 表达与几种免疫细胞、趋化因子和免疫调节剂相关。我们发现与 LUAD 的发展和预后相关，为进一步的研究奠定了基础，特别是在免疫治疗方面。
