Department of Veterinary Parasitology, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, India.
Department of Veterinary Pathology, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, India.
Acta Parasitol. 2021 Sep;66(3):899-906. doi: 10.1007/s11686-021-00348-w. Epub 2021 Mar 9.
Hydatid disease is one of the neglected and challenging (for diagnosis as well as for treatment) parasitic diseases. Along with adverse effect on animal's health leading to production losses, hydatidosis is also associated with huge economic losses. The present study was envisaged with an aim to assess the phylogeny and pathological changes due to natural hydatid cysts in lungs and liver of slaughtered buffaloes in north India.
A total of 137 slaughtered buffaloes intended for human consumption were screened for the presence of cysts. The retrieved cysts were confirmed molecularly based on the amplification of mitochondrial cytochrome oxidase 1 gene (mtCO1), exhibiting a product size of approximately 446 bp. The samples collected from infected lungs and liver were subjected to histopathological examination.
The hydatid cysts were recorded in 25 (18.2%) animals. Phylogenetic analysis revealed the isolated strain to be closely related to Echinococcus granulosus sensu stricto (G1) genotype. The nucleotide diversity (π) obtained was 0.014685, whereas, Tajima's D was negative (- 2.796053), which indicated purifying selection or recent population expansion. Histopathologically, in the infected lungs, fibrosis and inflammatory reaction comprising of mononuclear cells and fibroblasts around the thick coat of granulation tissue were observed. Marked calcified masses and necrosis were also observed in the calcified cysts. However, in case of infected livers, Kupffer cell hyperplasia, degeneration of hepatocytes, fibrosis and inflammatory cells were most commonly observed around the hydatid cysts.
The findings of the present study are of significant veterinary and medical importance owing to economic and public health impact of G1 genotype of E. granulosus.
包虫病是一种被忽视的具有挑战性的寄生虫病（无论是诊断还是治疗）。除了对动物健康造成不良影响导致生产损失外，包虫病还会造成巨大的经济损失。本研究旨在评估印度北部屠宰水牛肺部和肝脏中自然包虫囊肿引起的系统发育和病理变化。
对 137 头拟供人类食用的屠宰水牛进行筛查，以确定是否存在囊肿。基于线粒体细胞色素氧化酶 1 基因（mtCO1）的扩增，从回收的囊肿中得到分子证实，扩增产物大小约为 446 bp。从感染的肺和肝采集的样本进行组织病理学检查。
在 25 头（18.2%）动物中记录到包虫囊肿。系统发育分析显示，分离株与细粒棘球绦虫（Echinococcus granulosus sensu stricto）G1 基因型密切相关。获得的核苷酸多样性（π）为 0.014685，而 Tajima 的 D 为负值（-2.796053），这表明存在纯化选择或近期种群扩张。组织病理学检查显示，在感染的肺部，厚肉芽组织周围有纤维化和炎症反应，包括单核细胞和成纤维细胞。在钙化囊肿中还观察到明显的钙化肿块和坏死。然而，在感染的肝脏中，最常见的是围绕包虫囊肿的枯否细胞增生、肝细胞变性、纤维化和炎症细胞。
鉴于细粒棘球绦虫 G1 基因型对经济和公共卫生的影响，本研究的发现具有重要的兽医和医学意义。
