Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125001, India.
Department of Veterinary Parasitology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125001, India.
Acta Parasitol. 2022 Sep;67(3):1237-1245. doi: 10.1007/s11686-022-00564-y. Epub 2022 May 26.
Porcine cysticercosis is a neglected zoonotic disease of significant veterinary and medical importance owing to its economic impact and public health significance. The present study aimed at genetic characterization of Taenia solium metacestodes in slaughtered pigs of Haryana (North India).
A total of 213 (160 and 53 from Chandigarh and Hisar, respectively) slaughtered pigs intended for human consumption were screened for the presence of T. solium metacestodes. The retrieved metacestodes were confirmed molecularly based on the partial amplification of mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. Evolutionary divergence, haplotype and nucleotide diversities and neutrality indices of the retrieved isolates were also assessed.
Out of the 213 pigs, 2 (0.94%) revealed the presence of metacestodes involving 1 pig each from Chandigarh (0.62%) and Hisar (1.9%). The sequences obtained after custom sequencing were submitted to GenBank under the accession numbers LC661682-83. The present study haplotype clustered with haplotypes of Asian origin and showed variation from other haplotypes by 1-23 mutational steps. However, the present study isolates also showed nucleotide polymorphisms (A198T, A199G, A201T, G204A, T206A, C210T, T212G, T213A, T216G/A, T217C, T221C, C524T, G994A) at different positions, which indicated the presence of sub-lineages. Low nucleotide diversity (π = 0.020) and negative value of Tajima's D (- 1.304) observed for the haplotypes under consideration was indicative of purifying selection and recent population expansion.
Our study confirms the circulation of T. solium Asian genotype (with distinct sub-lineages) in study area and recommends strict control measures to contain the zoonotic disease.
猪囊尾蚴病是一种被忽视的动物源性人畜共患疾病，具有重要的兽医和医学意义，因为它对经济有影响，对公共卫生也很重要。本研究旨在对印度哈里亚纳邦（印度北部）屠宰猪的猪囊尾蚴进行遗传特征分析。
共筛查了 213 头（分别来自昌迪加尔和哈里亚纳的 160 头和 53 头）用于人类消费的屠宰猪，以检测是否存在猪带绦虫囊尾蚴。基于线粒体细胞色素 c 氧化酶亚单位 1（CO1）基因的部分扩增，对回收的囊尾蚴进行了分子确认。还评估了回收分离株的进化分歧、单倍型和核苷酸多样性以及中性指数。
在 213 头猪中，有 2 头（0.94%）检出囊尾蚴，其中 1 头来自昌迪加尔（0.62%），另 1 头来自哈里亚纳（1.9%）。定制测序后获得的序列已在 GenBank 中提交，登录号为 LC661682-83。本研究的单倍型与亚洲起源的单倍型聚类，并与其他单倍型有 1-23 个突变步骤的差异。然而，本研究的分离株也显示出核苷酸多态性（A198T、A199G、A201T、G204A、T206A、C210T、T212G、T213A、T216G/A、T217C、T221C、C524T、G994A）在不同位置，表明存在亚系。考虑到所考虑的单倍型的核苷酸多样性较低（π=0.020）和 Tajima's D 的负值（-1.304）表明存在纯化选择和近期种群扩张。
本研究证实了在研究区域存在猪带绦虫亚洲基因型（具有明显的亚系）的循环，并建议采取严格的控制措施来控制人畜共患病。
