Shafiei R, Ghatee M A, Jafarzadeh F, Javanshir Z, Karamian M
Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
J Helminthol. 2019 Jul 23;94:e64. doi: 10.1017/S0022149X19000579.
Despite several studies conducted to determine the genotypes of cystic echinococcosis (CE) agents in humans and other intermediate hosts, the relationship between parasite genotype and clinical presentation of hydatidosis is yet to be well defined. The aim of this study was to compare the genotypes/haplotypes of CE agents of human extra-hepatopulmonary hydatid cysts and common hydatid cysts of the liver. A comparative analysis was carried out between partial cox1 sequences of ten extra-hepatopulmonary hydatid cysts, two liver cysts and reliable sequences from the GenBank database. All the studied hydatid cysts had the Echinococcus granulosus sensu stricto (G1-G2-G3) genotypes. The liver CE cysts were caused by common G1 genotype, while six of the extra-hepatopulmonary cysts had genotypes different from common G1 cysts. The sequences of these six isolates were identical to the G2 and G3 genotypes of E. granulosus sensu stricto; the kidney and peritoneum cysts and most of the brain cysts were identified as G2 genotype, while G3 genotype was only reported in a cyst belonging to the pelvic region. Given the observed differences between the sequences of hydatid cysts, it seems that the replacement of hydatid cysts in organs other than the liver and lungs can be related to their genotypes and probably intra-genotypic characteristics. It was hypothesized that in each geographic area, less frequent genotypes were likely to be more consistent with placement in the host's unusual organs.
尽管已经开展了多项研究来确定人类及其他中间宿主中囊型包虫病（CE）病原体的基因型，但包虫病的寄生虫基因型与临床表现之间的关系仍未明确界定。本研究的目的是比较人类肝外肺外包虫囊肿与常见肝包虫囊肿的CE病原体的基因型/单倍型。对10个肝外肺外包虫囊肿、2个肝囊肿的部分cox1序列以及来自GenBank数据库的可靠序列进行了比较分析。所有研究的包虫囊肿均具有狭义细粒棘球绦虫（G1-G2-G3）基因型。肝CE囊肿由常见的G1基因型引起，而6个肝外肺外囊肿的基因型与常见的G1囊肿不同。这6个分离株的序列与狭义细粒棘球绦虫的G2和G3基因型相同；肾囊肿和腹膜囊肿以及大多数脑囊肿被鉴定为G2基因型，而G3基因型仅在一个盆腔区域的囊肿中被报道。鉴于观察到的包虫囊肿序列之间的差异，似乎肝和肺以外器官中的包虫囊肿替代可能与其基因型以及可能的基因型内特征有关。据推测，在每个地理区域，较罕见的基因型可能更符合在宿主异常器官中的定位。
