Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Mass Spectrometry Applications & Development, SpectraMass Ltd, Campinas, São Paulo, Brazil.
Methods Mol Biol. 2021;2259:153-165. doi: 10.1007/978-1-0716-1178-4_9.
Proteomic tools are especially useful when it comes to investigating complex samples such as human blood plasma, in which protein quantities can span across up to ten orders of magnitude. Ultra definition mass spectrometry, in combination with two-dimensional liquid chromatography, provides better coverage of complex proteomes and allows for better control of collision energy, keeping the fragmentation benefits of high collision energy associated with drift time measurements from ion mobility separation. Here, we present a protocol to assist in the identification of proteins in human blood plasma and other similar samples with a large dynamic range.
当涉及到研究复杂样本（如人类血浆）时，蛋白质组学工具特别有用，因为这些样本中的蛋白质数量可以跨越多达十个数量级。超高分辨质谱与二维液相色谱联用，可以更好地覆盖复杂的蛋白质组，并更好地控制碰撞能，保持与离子淌度分离相关的高碰撞能所带来的碎片化优势。在这里，我们提出了一个方案，以协助鉴定人类血浆和其他具有大动态范围的类似样本中的蛋白质。
