Department of Pharmaceutics, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt.
Int J Nanomedicine. 2021 Mar 2;16:1789-1804. doi: 10.2147/IJN.S298900. eCollection 2021.
SARS-COVID-2 has recently been one of the most life-threatening problems which urgently needs new therapeutic antiviral agents, especially those of herbal origin.
The study aimed to load acaciin (ACA) into the new self-assembled nanofibers (NFs) followed by investigating their possible antiviral effect against bovine coronavirus (BCV) as a surrogate model for SARS-COV-2.
ACA was identified using H-NMR and DEPT-Q C-NMR spectroscopy, the molecular docking study was performed using Autodock 4 and a modification of the traditional solvent injection method was applied for the synthesis of the biodegradable NFs. Different characterization techniques were used to inspect the formation of the NFs, which is followed by antiviral investigation against BCV as well as MTT assay using MDBK cells.
Core/shell NFs, ranging between 80-330 nm with tiny thorn-like branches, were formed which attained an enhanced encapsulation efficiency (97.5 ± 0.53%, P<0.05) and a dual controlled release (a burst release of 65% at 1 h and a sustained release up to >24 h). The antiviral investigation of the formed NFs revealed a significant inhibition of 98.88 ± 0.16% (P<0.05) with IC of 12.6 µM against BCV cells.
The results introduced a new, time/cost-saving strategy for the synthesis of biodegradable NFs without the need for electric current or hazardous cross-linking agents. Moreover, it provided an innovative avenue for the discovery of drugs of herbal origin for the fight against SARS-CoV-2 infection.
SARS-COV-2 是目前最具威胁生命的问题之一，急需新的治疗性抗病毒药物，尤其是源自草药的药物。
本研究旨在将阿拉伯聚糖（ACA）载入新型自组装纳米纤维（NFs）中，然后研究其对牛冠状病毒（BCV）的可能抗病毒作用，BCV 可作为 SARS-COV-2 的替代模型。
使用 H-NMR 和 DEPT-Q C-NMR 光谱学鉴定 ACA，使用 Autodock 4 进行分子对接研究，并采用传统溶剂注入法的改良方法合成可生物降解的 NFs。使用不同的表征技术检查 NFs 的形成，然后对其进行抗病毒研究，包括对 BCV 的抑制作用以及使用 MDBK 细胞进行 MTT 测定。
形成了 80-330nm 之间的核/壳 NFs，带有微小的刺状分支，实现了 97.5±0.53%（P<0.05）的增强包封效率和双重控制释放（1 小时内 65%的突释释放和持续释放长达>24 小时）。形成的 NFs 的抗病毒研究显示，对 BCV 细胞的抑制率为 98.88±0.16%（P<0.05），IC 为 12.6µM。
该结果提出了一种新的、节省时间/成本的合成可生物降解 NFs 的策略，无需电流或危险的交联剂。此外，它为发现源自草药的抗 SARS-CoV-2 感染药物提供了新途径。
