Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
In Vitro Cell Dev Biol Anim. 2021 Mar;57(3):264-271. doi: 10.1007/s11626-021-00554-w. Epub 2021 Mar 10.
Bifenthrin is one of the most commonly used synthetic pyrethroid insecticides. It targets the nervous system of insects, mainly acting on sodium channels in nerve cell membranes. The high use of bifrenthrin may lead to an increase in pest insect resistance. Additionally, there are only a few studies describing its cytotoxic action. A series of bioassays were carried out, and the results showed that bifenthrin has a significant ability to induce DNA damage and the inhibition of viability in Spodoptera frugiperda (Sf9) cells. Monodansylcadaverine staining and transmission electron microscope assays were used to observe significant levels of autophagosomes and mitochondrial dysfunction in the cytoplasm. Additionally, western blot analysis showed an upregulation in LC3-II and beclin-1 protein expression and a downregulation in p62 expression, which contributed to the cytotoxic effect of bifenthrin on Sf9 cells. Overall, bifenthrin significantly impacts the viability of Sf9 cells by inducing DNA damage and autophagy. These results provide a theoretical basis for understanding bifrenthin's mechanism of cytotoxicity.
联苯菊酯是最常用的合成拟除虫菊酯类杀虫剂之一。它作用于昆虫的神经系统，主要作用于神经细胞膜上的钠离子通道。高浓度使用联苯菊酯可能会导致害虫的抗药性增加。此外，仅有少数研究描述了它的细胞毒性作用。我们进行了一系列的生物测定，结果表明联苯菊酯具有显著的诱导 DNA 损伤和抑制 Sf9 细胞活力的能力。单丹磺酰尸胺染色和透射电子显微镜检测结果表明细胞质中自噬体和线粒体功能障碍的水平显著升高。此外，Western blot 分析显示 LC3-II 和 beclin-1 蛋白表达上调，p62 表达下调，这有助于联苯菊酯对 Sf9 细胞的细胞毒性作用。总的来说，联苯菊酯通过诱导 DNA 损伤和自噬显著影响 Sf9 细胞的活力。这些结果为理解联苯菊酯的细胞毒性机制提供了理论依据。
