Appl Opt. 2021 Feb 1;60(4):815-822. doi: 10.1364/AO.410993.
Digital holographic microscopy (DHM) is a technique that has high potential for analyzing biological samples and has been successfully applied to the study of cells and cell lines providing information about important parameters such as refractive index, morphology, and dry mass, among others; it has also found applicability to study the effects of therapeutic treatments. Finding the size and shape of cells is important since they tend to change in the presence of some pathologies. In this research work, we obtain the morphology thickness and refractive index of the A375 melanoma cell line through a slight tilting of the cell in a DHM setup. Further, the development of a novel mathematical expression based on this tilt and in the optical phase difference is presented. We show images of melanoma cells with the refractive index information included, and their morphology thickness as rendered from the holographic phase maps recorded with DHM.
数字全息显微镜（DHM）是一种具有分析生物样本潜力的技术，已成功应用于细胞和细胞系的研究，提供了有关折射率、形态和干质量等重要参数的信息；它也已被应用于研究治疗效果。找到细胞的大小和形状很重要，因为在某些病理情况下它们往往会发生变化。在这项研究工作中，我们通过在 DHM 装置中稍微倾斜细胞来获得 A375 黑色素瘤细胞系的形态厚度和折射率。此外，还提出了一种基于这种倾斜和光学相位差的新数学表达式。我们展示了包含折射率信息的黑色素瘤细胞的图像，以及从用 DHM 记录的全息相位图渲染的细胞形态厚度。
