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优化透射断层衍射显微镜中的样品照明扫描

Optimizing sample illumination scanning in transmission tomographic diffractive microscopy.

作者信息

Taddese Asemare Mengistie, Verrier Nicolas, Debailleul Matthieu, Courbot Jean-Baptiste, Haeberlé Olivier

出版信息

Appl Opt. 2021 Feb 20;60(6):1694-1704. doi: 10.1364/AO.417061.

DOI:10.1364/AO.417061
PMID:33690516
Abstract

Due to the sequential nature of data acquisition, it is preferable to limit the number of illuminations to be used in tomographic diffractive microscopy experiments, especially if fast imaging is foreseen. On the other hand, for high-quality, high-resolution imaging, the Fourier space has to be optimally filled. Up to now, the problem of optimal Fourier space filling has not been investigated in itself. In this paper, we perform a comparative study to analyze the effect of sample scanning patterns on Fourier space filling for a transmission setup. Optical transfer functions for several illumination patterns are studied. Simulation as well as experiments are conducted to compare associated image reconstructions. We found that 3D uniform angular sweeping best fills the Fourier space, leading to better quality images.

摘要

由于数据采集的顺序性,在断层衍射显微镜实验中,最好限制所使用的照明次数,尤其是在预期进行快速成像的情况下。另一方面,为了获得高质量、高分辨率的成像,傅里叶空间必须得到最佳填充。到目前为止,最佳傅里叶空间填充问题本身尚未得到研究。在本文中,我们进行了一项比较研究,以分析样品扫描模式对透射设置中傅里叶空间填充的影响。研究了几种照明模式的光学传递函数。通过模拟和实验来比较相关的图像重建。我们发现三维均匀角扫描能最好地填充傅里叶空间,从而得到质量更好的图像。

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Optimizing sample illumination scanning in transmission tomographic diffractive microscopy.优化透射断层衍射显微镜中的样品照明扫描
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3D differential interference contrast microscopy using polarisation-sensitive tomographic diffraction microscopy.基于偏振敏感层析衍射显微镜的 3D 微分干涉对比显微镜。
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