Ossowski Paweł, Kuś Arkadiusz, Krauze Wojciech, Tamborski Szymon, Ziemczonok Michał, Kuźbicki Łukasz, Szkulmowski Maciej, Kujawińska Małgorzata
Warsaw University of Technology, Institute of Micromechanics and Photonics, Boboli 8 street, Warsaw, 02-525, Poland.
Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, Grudziadzka 5, 87-100 Toruń, Poland.
Biomed Opt Express. 2022 Oct 21;13(11):5971-5988. doi: 10.1364/BOE.468046. eCollection 2022 Nov 1.
We present a holographic tomography technique in which the projections are acquired using both wavelength and illumination scanning in the near-infrared region. We show how to process the acquired data to obtain correct values of three-dimensional refractive index distributions in both single-wavelength and multi-wavelength data acquisition schemes and how to properly account for the dispersion of the sample. We perform numerical and experimental comparisons of different illumination scenarios to determine the most efficient measurement protocol. We show that the multi-wavelength protocol is advantageous in terms of signal-to-noise ratio and contrast-to-noise ratio over single-wavelength protocols, even for the same number of projections used for reconstructions. Finally, we show that this approach is suitable for providing high-quality refractive index distributions of relatively thick colon cancer samples.
我们提出了一种全息断层扫描技术，该技术在近红外区域通过波长和照明扫描来获取投影。我们展示了如何处理采集到的数据，以在单波长和多波长数据采集方案中获得三维折射率分布的正确值，以及如何正确考虑样品的色散。我们对不同的照明场景进行了数值和实验比较，以确定最有效的测量方案。我们表明，即使对于用于重建的相同投影数量，多波长方案在信噪比和对比度噪声比方面也比单波长方案更具优势。最后，我们表明这种方法适用于提供相对较厚的结肠癌样本的高质量折射率分布。
