来自HIV携带者的免疫球蛋白制剂在体外预防HIV复制的效果。

Efficacy of an immunoglobulin preparation from HIV carriers in preventing HIV replication in vitro.

作者信息

Tochikura T S, Nakashima H, Uemura Y, Goto T, Suyama T, Kobayashi N, Yamamoto N

机构信息

Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.

出版信息

Vox Sang. 1988;54(3):138-43. doi: 10.1111/j.1423-0410.1988.tb03888.x.

DOI:10.1111/j.1423-0410.1988.tb03888.x

PMID:3369135

Abstract

Immunoglobulin samples (HIV-Ig) were prepared by cold ethanol fractionation of human plasma containing antibody against human immunodeficiency virus (HIV). The ability to prevent viral spreading was studied using either human T-cell leukemia virus type I (HTLV-I)-carrying MT-4 cells or in a coculture system using MOLT-4 cells and virus-producing MOLT-4/HIV HTLV-IIIB cells. Treatment of HIV-infected MT-4 cells with HIV-Ig effectively blocked the appearance of antigens of HIV and the virus-induced cytopathic effect. HIV-Ig blocked multinucleated giant cell formation in the MOLT-4 and MOLT-4/HIV HTLV-IIIB coculture system.

摘要

免疫球蛋白样本（HIV-Ig）通过对含抗人类免疫缺陷病毒（HIV）抗体的人血浆进行冷乙醇分级分离制备。使用携带I型人类T细胞白血病病毒（HTLV-I）的MT-4细胞或在使用MOLT-4细胞和产生病毒的MOLT-4/HIV HTLV-IIIB细胞的共培养系统中研究预防病毒传播的能力。用HIV-Ig处理感染HIV的MT-4细胞可有效阻断HIV抗原的出现和病毒诱导的细胞病变效应。HIV-Ig在MOLT-4和MOLT-4/HIV HTLV-IIIB共培养系统中阻断多核巨细胞的形成。