在齐多夫定和中和抗体存在的情况下，人类免疫缺陷病毒1型的细胞间传播
Cell-to-cell transmission of human immunodeficiency virus type 1 in the presence of azidothymidine and neutralizing antibody.
作者信息
Gupta P, Balachandran R, Ho M, Enrico A, Rinaldo C
机构信息
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
出版信息
J Virol. 1989 May;63(5):2361-5. doi: 10.1128/JVI.63.5.2361-2365.1989.
Abstract
Very few peripheral blood lymphocytes of seropositive individuals are presumably actively infected with human immunodeficiency virus type 1 (HIV-1). During coculture of lymphocytes of a seropositive individual with mitogen-stimulated normal peripheral blood lymphocytes, the number of infected cells becomes amplified such that detectable HIV-1 is produced. We report here that in addition to transmission by extracellular virus, cell-to-cell transmission is responsible for spreading HIV-1 infection from infected to uninfected cells. Azidothymidine and virus-neutralizing antibody had no effect on cell-to-cell transmission of HIV-1. Monoclonal antibodies to the CD4 receptor, but not to the CD3 receptor, prevented cell-to-cell transmission, which suggests that CD4 receptor-mediated cell fusion is involved in cell-to-cell transmission. Spread of infection in a cell-to-cell manner may be important in development of drug therapies for HIV-1 infection.
摘要
血清反应阳性个体的外周血淋巴细胞中，可能只有极少数被1型人类免疫缺陷病毒（HIV-1）主动感染。在血清反应阳性个体的淋巴细胞与有丝分裂原刺激的正常外周血淋巴细胞共培养过程中，感染细胞的数量会增加，从而产生可检测到的HIV-1。我们在此报告，除了通过细胞外病毒传播外，细胞间传播也导致HIV-1感染从感染细胞扩散到未感染细胞。叠氮胸苷和病毒中和抗体对HIV-1的细胞间传播没有影响。针对CD4受体而非CD3受体的单克隆抗体可阻止细胞间传播，这表明CD4受体介导的细胞融合参与了细胞间传播。以细胞间方式进行的感染传播可能对HIV-1感染的药物治疗发展具有重要意义。
相似文献
1
Cell-to-cell transmission of human immunodeficiency virus type 1 in the presence of azidothymidine and neutralizing antibody.在齐多夫定和中和抗体存在的情况下，人类免疫缺陷病毒1型的细胞间传播
J Virol. 1989 May;63(5):2361-5. doi: 10.1128/JVI.63.5.2361-2365.1989.
2
In vitro synthesis of human immunodeficiency virus-specific antibodies in peripheral blood lymphocytes of infants.婴儿外周血淋巴细胞中人类免疫缺陷病毒特异性抗体的体外合成
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7532-6. doi: 10.1073/pnas.86.19.7532.
3
4
Use of cryopreserved normal peripheral blood lymphocytes for isolation of human immunodeficiency virus from seropositive men.使用冷冻保存的正常外周血淋巴细胞从血清学阳性男性中分离人类免疫缺陷病毒。
J Clin Microbiol. 1988 Mar;26(3):595-7. doi: 10.1128/jcm.26.3.595-597.1988.
5
Efficient primary isolation of human immunodeficiency virus.人类免疫缺陷病毒的高效初次分离
Med J Aust. 1989 Apr 3;150(7):376-7. doi: 10.5694/j.1326-5377.1989.tb136528.x.
6
Human immunodeficiency virus DNA is present in a high percentage of CD4+ lymphocytes of seropositive individuals.人类免疫缺陷病毒DNA存在于血清反应阳性个体的高比例CD4 +淋巴细胞中。
J Infect Dis. 1991 Sep;164(3):470-5. doi: 10.1093/infdis/164.3.470.
7
Differential requirements for HIV-1 replication in naive and memory CD4 T cells from asymptomatic HIV-1 seropositive carriers and AIDS patients.无症状HIV-1血清阳性携带者和艾滋病患者的初始和记忆CD4 T细胞中HIV-1复制的差异需求。
Clin Exp Immunol. 1993 Feb;91(2):241-8. doi: 10.1111/j.1365-2249.1993.tb05890.x.
8
Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals.在所有血清学阳性的有症状和无症状个体中均检测到1型人类免疫缺陷病毒。
J Clin Microbiol. 1990 Jan;28(1):16-9. doi: 10.1128/jcm.28.1.16-19.1990.
9
Ex vivo expansion of CD4 lymphocytes from human immunodeficiency virus type 1-infected persons in the presence of combination antiretroviral agents.在联合抗逆转录病毒药物存在的情况下，对来自1型人类免疫缺陷病毒感染者的CD4淋巴细胞进行体外扩增。
J Infect Dis. 1995 Jul;172(1):88-96. doi: 10.1093/infdis/172.1.88.
10
Quantitation of HIV: correlation with clinical, virological, and immunological status.人类免疫缺陷病毒定量：与临床、病毒学及免疫学状态的相关性
J Med Virol. 1991 Sep;35(1):65-9. doi: 10.1002/jmv.1890350114.
引用本文的文献
1
The role of tunneling nanotubes during early stages of HIV infection and reactivation: implications in HIV cure.隧道纳米管在HIV感染和再激活早期阶段的作用：对HIV治愈的影响。
NeuroImmune Pharm Ther. 2023 Jan 4;2(2):169-186. doi: 10.1515/nipt-2022-0015. eCollection 2023 Jun.
2
Cellular Targets of HIV-1 Protease: Just the Tip of the Iceberg?HIV-1 蛋白酶的细胞靶标：仅仅是冰山一角？
Viruses. 2023 Mar 9;15(3):712. doi: 10.3390/v15030712.
3
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin.白细胞介素-27 通过 BST-2/ tetherin 对外周血单个核细胞中的 HIV-1 感染产生不同的影响。
J Virol. 2023 Jan 31;97(1):e0175222. doi: 10.1128/jvi.01752-22. Epub 2023 Jan 5.
4
The HIV Env Glycoprotein Conformational States on Cells and Viruses.HIV Env 糖蛋白在细胞和病毒上的构象状态。
mBio. 2022 Apr 26;13(2):e0182521. doi: 10.1128/mbio.01825-21. Epub 2022 Mar 24.
5
A Replication-Competent HIV Clone Carrying GFP-Env Reveals Rapid Env Recycling at the HIV-1 T Cell Virological Synapse.带有 GFP-Env 的复制型 HIV 克隆揭示了 HIV-1 T 细胞病毒学突触处的Env 快速回收。
Viruses. 2021 Dec 25;14(1):38. doi: 10.3390/v14010038.
6
The infectivity of progeny adenovirus in the presence of neutralizing antibody.存在中和抗体时，子代腺病毒的感染力。
J Gen Virol. 2021 Apr;102(4). doi: 10.1099/jgv.0.001590.
7
Viral cell-to-cell spread: Conventional and non-conventional ways.病毒细胞间传播：传统和非传统方式。
Adv Virus Res. 2020;108:85-125. doi: 10.1016/bs.aivir.2020.09.002. Epub 2020 Sep 29.
8
Equid Herpesvirus-1 Exploits the Extracellular Matrix of Mononuclear Cells to Ensure Transport to Target Cells.马疱疹病毒1型利用单核细胞的细胞外基质确保向靶细胞的转运。
iScience. 2020 Oct 23;23(10):101615. doi: 10.1016/j.isci.2020.101615. Epub 2020 Sep 28.
9
On the efficiency of HIV transmission: Insights through discrete time HIV models.关于 HIV 传播效率：离散时间 HIV 模型的研究进展。
PLoS One. 2019 Sep 18;14(9):e0222574. doi: 10.1371/journal.pone.0222574. eCollection 2019.
10
Negative and Positive Selection Pressure During Sexual Transmission of Transmitted Founder HIV-1.性传播传播起始 HIV-1 过程中的正负选择压力
Front Immunol. 2019 Jul 10;10:1599. doi: 10.3389/fimmu.2019.01599. eCollection 2019.
本文引用的文献
1
2
Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster.水痘和带状疱疹来源的产生包涵体的病原体的体外连续传代培养
Proc Soc Exp Biol Med. 1953 Jun;83(2):340-6. doi: 10.3181/00379727-83-20354.
3
Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.从旧金山艾滋病患者中分离淋巴细胞病变逆转录病毒。
Science. 1984 Aug 24;225(4664):840-2. doi: 10.1126/science.6206563.
4
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.从艾滋病患者和有患艾滋病风险的人群中频繁检测和分离出细胞病变逆转录病毒（HTLV-III）。
Science. 1984 May 4;224(4648):500-3. doi: 10.1126/science.6200936.
5
Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).从一名有获得性免疫缺陷综合征（艾滋病）风险的患者体内分离出一种嗜T淋巴细胞逆转录病毒。
Science. 1983 May 20;220(4599):868-71. doi: 10.1126/science.6189183.
6
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.CD4（T4）抗原是艾滋病逆转录病毒受体的重要组成部分。
Nature. 1984;312(5996):763-7. doi: 10.1038/312763a0.
7
Immune sera and antiglycoprotein monoclonal antibodies inhibit in vitro cell-to-cell spread of pathogenic rabies viruses.免疫血清和抗糖蛋白单克隆抗体可在体外抑制致病性狂犬病病毒的细胞间传播。
J Virol. 1987 Oct;61(10):3314-8. doi: 10.1128/JVI.61.10.3314-3318.1987.
8
Development of antiretroviral therapy for the acquired immunodeficiency syndrome and related disorders. A progress report.
N Engl J Med. 1987 Feb 26;316(9):557-64. doi: 10.1056/NEJM198702263160925.
9
Use of cryopreserved normal peripheral blood lymphocytes for isolation of human immunodeficiency virus from seropositive men.使用冷冻保存的正常外周血淋巴细胞从血清学阳性男性中分离人类免疫缺陷病毒。
J Clin Microbiol. 1988 Mar;26(3):595-7. doi: 10.1128/jcm.26.3.595-597.1988.
10
The efficacy of azidothymidine (AZT) in the treatment of patients with AIDS and AIDS-related complex. A double-blind, placebo-controlled trial.叠氮胸苷（AZT）治疗艾滋病及艾滋病相关综合征患者的疗效。一项双盲、安慰剂对照试验。
N Engl J Med. 1987 Jul 23;317(4):185-91. doi: 10.1056/NEJM198707233170401.
Zotero 插件