Matsuyama T, Hamamoto Y, Yoshida T, Kido Y, Kobayashi S, Kobayashi N, Yamamoto N
Department of Virology and Parasitology, Yamaguchi University School of Medicine.
Jpn J Cancer Res. 1988 Feb;79(2):156-9. doi: 10.1111/j.1349-7006.1988.tb01571.x.
The effect of culture supernatant of MT-2 cells on human immunodeficiency virus (HIV)-producing cells, MOLT-4/HIVHTLV-IIIB cells, was examined. As compared to the effect on MOLT-4 cells, parent cells not infected with HIV, a selective cytotoxic/cytostatic effect on MOLT-4/HIVHTLV-IIIB cells was observed 4 days after treatment with up to 640-fold-diluted MT-2 supernatant. Furthermore, under similar conditions, a 2- to 6-fold increase in the number of HIV particles was detected in the culture of MOLT-4/HIVHTLV-IIIB cells 6 hr after treatment. Complete blocking of these effects by anti-lymphotoxin monoclonal antibody, but not by anti-tumor necrosis factor antibody, indicates that these effects of MT-2 supernatant on MOLT-4/HIVHTLV-IIIB cells are attributable to a lymphotoxin-related cytotoxic factor.
研究了MT-2细胞的培养上清液对产生人类免疫缺陷病毒（HIV）的细胞，即MOLT-4/HIVHTLV-IIIB细胞的影响。与对未感染HIV的亲本细胞MOLT-4细胞的影响相比，在用高达640倍稀释的MT-2上清液处理4天后，观察到对MOLT-4/HIVHTLV-IIIB细胞有选择性细胞毒性/细胞生长抑制作用。此外，在类似条件下，处理6小时后，在MOLT-4/HIVHTLV-IIIB细胞培养物中检测到HIV颗粒数量增加了2至6倍。抗淋巴毒素单克隆抗体可完全阻断这些作用，但抗肿瘤坏死因子抗体则不能，这表明MT-2上清液对MOLT-4/HIVHTLV-IIIB细胞的这些作用归因于一种与淋巴毒素相关的细胞毒性因子。
