Wei Jiahui, Zhang Xuefeng, Pan Huihao, He Song, Yuan Bao, Liu Qing, Zhang Jiabao, Ding Yu
Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.
Oncol Lett. 2021 Apr;21(4):332. doi: 10.3892/ol.2021.12593. Epub 2021 Feb 25.
Eupafolin is a flavonoid extracted from common sage. Previous studies have reported that Eupafolin has antioxidant, anti-inflammatory, and anti-tumor effects. However, its role in breast cancer remains unclear. The present study investigated the effects and underlying mechanism of action of Eupafolin using breast cancer cell lines. The effects of Eupafolin on breast cancer cell proliferation, migration, apoptosis and the cell cycle were determined. Cell viability and Transwell assays, reverse transcription-quantitative PCR, flow cytometry and western blot analysis were used in this study. The data showed that the proliferation, migration and invasion ability of EO771 cells treated with Eupafolin was significantly decreased, and the apoptosis rate was increased compared with that of the control. The protein levels of Bax and cleaved caspase 3 increased, whereas that of Bcl-2 decreased. In addition, Eupafolin treatment also caused the proliferation of breast cancer cells to be arrested at the G/G phase. Furthermore, results from western blotting indicated that Eupafolin treatment decreased the protein levels of p-PI3K, p-Akt and p-mTOR. Taken together, the present findings demonstrate that Eupafolin has a significant inhibitory effect on the proliferation of EO771 cells, inhibits cell migration and invasion, and promotes cell apoptosis, thereby causing G/G phase arrest, at least partially through the PI3K/Akt/mTOR signaling pathway. Therefore, the findings provide novel insights regarding the use of Eupafolin for the treatment of breast cancer.
灯盏乙素是从鼠尾草中提取的一种黄酮类化合物。先前的研究报道灯盏乙素有抗氧化、抗炎和抗肿瘤作用。然而，其在乳腺癌中的作用仍不清楚。本研究使用乳腺癌细胞系研究了灯盏乙素的作用及其潜在作用机制。测定了灯盏乙素对乳腺癌细胞增殖、迁移、凋亡和细胞周期的影响。本研究采用细胞活力和Transwell实验、逆转录定量PCR、流式细胞术和蛋白质印迹分析。数据显示，与对照组相比，用灯盏乙素处理的EO771细胞的增殖、迁移和侵袭能力显著降低，凋亡率增加。Bax和裂解的caspase 3的蛋白质水平升高，而Bcl-2的蛋白质水平降低。此外，灯盏乙素处理还导致乳腺癌细胞的增殖停滞在G/G期。此外，蛋白质印迹结果表明，灯盏乙素处理降低了p-PI3K、p-Akt和p-mTOR的蛋白质水平。综上所述，本研究结果表明灯盏乙素对EO771细胞的增殖具有显著抑制作用，抑制细胞迁移和侵袭，并促进细胞凋亡，从而导致G/G期停滞，至少部分是通过PI3K/Akt/mTOR信号通路实现的。因此，这些发现为灯盏乙素用于治疗乳腺癌提供了新的见解。
