Gao Yan, Zhang Yi, Fan Yangyang
Function Testing Lab, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi P.R. China.
ICU Department, Shaanxi Provincial People's Hospital. Xi'an, Shaanxi P.R. China.
Iran J Basic Med Sci. 2019 Nov;22(11):1340-1346. doi: 10.22038/ijbms.2019.37748.8977.
Eupafolin, a major active component of ., has anti-inflammatory and anti-oxidant properties. Lipopolysaccharide (LPS) is responsible for myocardial depression. A line of evidences revealed that LPS induces autophagy in cardiomyocytes injury. This study aims to evaluate the effects of eupafolin on LPS-induced cardiomyocyte autophagy.
The effect of LPS on cell viability was examined by CCK-8. Autophagic protein 2 light chain 3 (LC3II), which was regulated by LPS and eupafolin, was examined using immunofluorescent staining. The expression levels of Beclin-1 and p62 were detected by western blotting. The effects of eupafolin on phosphatidylinositol-3-kinase/ protein kinase B/ mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway were also evaluated by western blotting and immunofluorescent staining.
Eupafolin pretreatment reduced the expression of LC3II and Beclin-1, whereas p62 was significant increased. In addition, eupafolin promoted expression of PI3K/AKT/mTOR signaling pathway and mTOR inhibitor rapamycin reversed the inhibitory effects on LPS-induced cardiomyocyte autophagy.
Eupafolin exerts anti-autophagy activity via activation of PI3K/AKT/mTOR signaling pathway.
欧前胡素是……的主要活性成分，具有抗炎和抗氧化特性。脂多糖（LPS）可导致心肌抑制。一系列证据表明，LPS可诱导心肌细胞损伤中的自噬。本研究旨在评估欧前胡素对LPS诱导的心肌细胞自噬的影响。
采用CCK-8检测LPS对细胞活力的影响。使用免疫荧光染色检测受LPS和欧前胡素调节的自噬蛋白2轻链3（LC3II）。通过蛋白质免疫印迹法检测Beclin-1和p62的表达水平。还通过蛋白质免疫印迹法和免疫荧光染色评估欧前胡素对磷脂酰肌醇-3-激酶/蛋白激酶B/雷帕霉素哺乳动物靶标（PI3K/AKT/mTOR）信号通路的影响。
欧前胡素预处理降低了LC3II和Beclin-1的表达，而p62显著增加。此外，欧前胡素促进了PI3K/AKT/mTOR信号通路的表达，mTOR抑制剂雷帕霉素逆转了对LPS诱导的心肌细胞自噬的抑制作用。
欧前胡素通过激活PI3K/AKT/mTOR信号通路发挥抗自噬活性。
