Departamento de Ciência e Tecnologia Agroindustrial, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil.
Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil.
Can J Microbiol. 2021 Apr;67(4):301-309. doi: 10.1139/cjm-2020-0256. Epub 2021 Mar 11.
The aims of this study were to evaluate the ability of isolated from a poultry slaughterhouse to form biofilm in the presence and absence of , and the effect of surface (stainless steel, polystyrene), temperature (7, 25, and 42 °C), and oxygen concentration (microaerophilic and aerobic conditions) on the formation of biofilm. The genes , , , , , , , , , , , and , related to biofilm formation by , were also investigated. All isolates formed biofilm on stainless steel and on polystyrene, in both aerobic and microaerophilic atmospheres, including temperatures not optimal for growth (7 and 25 °C), and biofilm also was formed in the presence of . In dual-species biofilm on stainless steel, biofilm formation was 2-6 log CFU·cm higher at 7 °C for all isolates, in comparison with monospecies biofilm. Ten genes (, , , , , , , , , and ) were detected in all isolates, but and were found in four and six isolates, respectively. The results obtained are of concern because the poultry isolates form biofilm in different conditions, which is enhanced in the presence of other biofilm formers, such as .
本研究的目的是评估从家禽屠宰场分离出的 形成生物膜的能力，以及在有和没有 的情况下，表面（不锈钢、聚苯乙烯）、温度（7、25 和 42°C）和氧浓度（微需氧和需氧条件）对生物膜形成的影响。还研究了与 生物膜形成相关的基因 、 、 、 、 、 、 、 、 、 和 。所有分离株均在有氧和微需氧条件下，在不锈钢和聚苯乙烯上形成生物膜，包括 生长非最佳温度（7 和 25°C），并且在存在 的情况下也形成生物膜。在不锈钢上的双物种生物膜中，与单物种生物膜相比，所有分离株在 7°C 时的生物膜形成量增加了 2-6 个对数 CFU·cm。所有分离株均检测到 10 个基因（ 、 、 、 、 、 、 、 和 ），但 和 分别在 4 个和 6 个分离株中发现。这些结果令人担忧，因为家禽 分离株在不同条件下形成生物膜，而在其他生物膜形成菌（如 ）存在的情况下，生物膜的形成会增强。
