Tsukuda Haruka, Akimoto Taiki, Fukikoshi Nona, Wada Resei, Sawai Jun
Department of Nutrition and Life Science, Faculty of Health and Medical Sciences, Kanagawa Institute of Technology, 1030 Shimo-Ogino, Atsugi 243-0292, Kanagawa, Japan.
Faculty of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-Ogino, Atsugi 243-0292, Kanagawa, Japan.
Membranes (Basel). 2021 Dec 29;12(1):43. doi: 10.3390/membranes12010043.
Methods to reuse large numbers of scallop shells from the harvesting regions of Japan are being explored. The major component of scallop shells is calcium carbonate (CaCO), which forms the powerful bactericidal agent, calcium oxide (CaO), when heated. Heated scallop shell powder (HSSP) exhibits strong and broad-spectrum antimicrobial activity against bacteria, fungi, and viruses. This study investigated the antibiofilm activity of HSSP against the biofilms of , which is the predominant species in campylobacteriosis. Biofilm samples of were prepared on 0.45 µm filter paper under microaerobic conditions. The HSSP treatment inactivated and eradicated biofilms. The resistance of biofilms to HSSP was significantly higher than that of the floating cells. Moreover, the antibiofilm activity of the HSSP treatment against biofilms was higher than that of NaOH treatment at the same pH. These results indicated that HSSP treatment is an effective method for controlling biofilms.
人们正在探索回收日本捕捞地区大量扇贝贝壳的方法。扇贝贝壳的主要成分是碳酸钙（CaCO），加热时会形成强效杀菌剂氧化钙（CaO）。加热后的扇贝贝壳粉（HSSP）对细菌、真菌和病毒具有强大的广谱抗菌活性。本研究调查了HSSP对弯曲杆菌病主要致病菌空肠弯曲菌生物膜的抗生物膜活性。在微需氧条件下，在0.45 µm滤纸上制备空肠弯曲菌生物膜样本。HSSP处理使空肠弯曲菌生物膜失活并根除。空肠弯曲菌生物膜对HSSP的抗性明显高于浮游细胞。此外，在相同pH值下，HSSP处理对空肠弯曲菌生物膜的抗生物膜活性高于NaOH处理。这些结果表明，HSSP处理是控制空肠弯曲菌生物膜的有效方法。
