Mazurkiewicz Ewa, Mrówczyńska Ewa, Simiczyjew Aleksandra, Nowak Dorota, Mazur Antonina J
Department of Cell Pathology, Faculty of Biotechnology, University of Wroclaw, Wrocław, Poland.
Methods Mol Biol. 2021;2265:47-63. doi: 10.1007/978-1-0716-1205-7_3.
In order to protrude within a dense tissue, tumor cells have to develop the ability to digest the extracellular matrix (ECM). Melanoma cells, similarly to other types of tumor cells, form invadopodia, membranous invaginations rich in filamentous actin and several other proteins including matrix metalloproteinases (MMPs). MMPs degrade ECM structural proteins such as collagens, fibronectin, or laminin. Here we describe an assay that allows the detection of gelatinase activity exhibited by tumor cells under 2D conditions and methods to present obtained data in both a quantitative and a qualitative manner.
为了在致密组织中突出，肿瘤细胞必须发展出消化细胞外基质（ECM）的能力。黑色素瘤细胞与其他类型的肿瘤细胞类似，会形成侵袭伪足，即富含丝状肌动蛋白和包括基质金属蛋白酶（MMPs）在内的其他几种蛋白质的膜内陷。MMPs可降解ECM结构蛋白，如胶原蛋白、纤连蛋白或层粘连蛋白。在此，我们描述了一种检测方法，可用于检测肿瘤细胞在二维条件下表现出的明胶酶活性，以及以定量和定性方式呈现所得数据的方法。
