Morici Michael, Lin Weitao, Gray Elin S
School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia.
Methods Mol Biol. 2021;2265:235-245. doi: 10.1007/978-1-0716-1205-7_18.
Circulating tumor cells (CTCs) are cancer cells shed by the primary tumor or its metastases that circulate in the peripheral blood. CTCs are potential seeds for metastases, and their detection may allow early uncovering of metastatic dissemination and disease prognostication. To fully ascertain the biomarker potential of melanoma CTCs, sensitive and reliable methods are required. Melanoma-specific transcript analysis has been widely utilized as a standard approach for measuring the presence of CTCs. Here we describe a method for the analysis of CTCs through the detection of specific transcripts in CTC-enriched fractions.
循环肿瘤细胞（CTCs）是由原发性肿瘤或其转移灶脱落进入外周血循环的癌细胞。CTCs是转移的潜在种子，对它们的检测可能有助于早期发现转移扩散和疾病预后评估。为了全面确定黑色素瘤CTCs的生物标志物潜力，需要灵敏且可靠的方法。黑色素瘤特异性转录本分析已被广泛用作检测CTCs存在的标准方法。在此，我们描述一种通过检测富集CTCs组分中的特异性转录本来分析CTCs的方法。
