Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital (MGH) Research Institute, MGH and Harvard Medical School (HMS), Boston, MA, USA.
Bristol Myers Squibb, Oncology Discovery, Cambridge, MA, USA.
Methods Mol Biol. 2021;2265:363-376. doi: 10.1007/978-1-0716-1205-7_26.
The lymph node microenvironment is extremely dynamic and responds to immune stimuli in the host by reprogramming immune, stromal, and endothelial cells. In normal physiological conditions, the lymph node will initiate an appropriate immune response to clear external threats that the host may experience. However, in metastatic disease, cancer cells often colonize local lymph nodes, disrupt immune function, and even leave the lymph node to create additional metastases. Understanding how cancer cells enter, colonize, survive, proliferate, and interact with other cell types in the lymph node is challenging. Here, we describe the use of photoconvertible fluorescent proteins to label and trace the fate of cancer cells once they enter the lymph node.
淋巴结微环境极其动态，并通过重新编程免疫细胞、基质细胞和内皮细胞来响应宿主的免疫刺激。在正常生理条件下，淋巴结将启动适当的免疫反应，以清除宿主可能面临的外部威胁。然而，在转移性疾病中，癌细胞经常定植于局部淋巴结，破坏免疫功能，甚至离开淋巴结以形成额外的转移灶。了解癌细胞如何进入、定植、存活、增殖以及与淋巴结中的其他细胞类型相互作用具有挑战性。在这里，我们描述了使用光转化荧光蛋白来标记和追踪癌细胞进入淋巴结后的命运。
