高通量鉴定黑素瘤中的 miRNA 靶相互作用：miR-CATCHv2.0 方法
High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0.
机构信息
Oncogenomics Unit, CRL-ISPRO, Pisa, Italy.
Institute of Clinical Physiology, CNR, Pisa, Italy.
出版信息
Methods Mol Biol. 2021;2265:487-512. doi: 10.1007/978-1-0716-1205-7_35.
MicroRNAs (miRNAs) can regulate the expression of potentially every transcript in the cell, and the definition of miRNA-target interactions is crucial to understand their role in all biological processes. However, the identification of the miRNAs that target a specific mRNA remains a challenge. Here, we describe an innovative method called miR-CATCHv2.0 for the high-throughput identification of the miRNA species bound to an RNA of interest. We also describe how this method can overcome the limitations of the current computational and experimental methods available in this field.
MicroRNAs (miRNAs) 可以调控细胞中潜在的每一个转录本的表达，miRNA 与靶标相互作用的定义对于理解它们在所有生物过程中的作用至关重要。然而，鉴定靶向特定 mRNA 的 miRNAs 仍然是一个挑战。在这里，我们描述了一种名为 miR-CATCHv2.0 的创新方法，用于高通量鉴定与感兴趣的 RNA 结合的 miRNA 种类。我们还描述了该方法如何克服该领域现有计算和实验方法的局限性。
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