Institute of Hygiene and National Consulting Laboratory for Hemolytic Uremic Syndrome (HUS), University Hospital Münster, Münster, Germany.
Methods Mol Biol. 2021;2291:87-97. doi: 10.1007/978-1-0716-1339-9_3.
Today, whole genome sequencing (WGS)-based typing is the gold standard approach to detect outbreaks of Shiga toxin-producing Escherichia coli (STEC) and to differentiate them from sporadic cases. Here, we describe an optimized protocol to efficiently determine the genome sequences of STEC using short read Illumina technology and provide information on helpful tools for the subsequent bioinformatic analysis.
如今，基于全基因组测序（WGS）的分型方法是检测产志贺毒素大肠杆菌（STEC）暴发并将其与散发病例区分开来的金标准方法。在这里，我们描述了一种优化的方案，该方案使用短读长 Illumina 技术来高效地确定 STEC 的基因组序列，并提供了有助于后续生物信息学分析的工具信息。
