长链非编码 RNA ADAMTS9-AS1 通过调节 miR-142-5p/CCND1 轴抑制前列腺癌的进展。
Long non-coding RNA ADAMTS9-AS1 inhibits the progression of prostate cancer by modulating the miR-142-5p/CCND1 axis.
机构信息
Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
出版信息
J Gene Med. 2021 May;23(5):e3331. doi: 10.1002/jgm.3331. Epub 2021 Apr 7.
BACKGROUND
Emerging evidence has implied the importance of long non-coding RNAs in cancer development, including prostate cancer (PCa). Bioinformatic analyses have identified that ADAMTS9-AS1 may play a role in cancer progression.
METHODS
A quantitative real-time polymerase chain reaction was conducted to measure ADAMTS9-AS1 expression level at messenger RNA level. In vitro functional analyses were performed to investigate cell behaviors status under different conditions. Moreover, rescue assays were conducted to explore the potential mechanisms of ADAMTS9-AS1 in PCa progression.
RESULTS
ADAMTS9-AS1 expression is down-regulated in PCa. Forcing ADAMTS9-AS1 expression impedes PCa cell proliferation via initiating cell apoptosis. Importantly, microRNA-142-5p (miR-142-5p) mimic and small-interfering RNA targeting cyclin D1 (CCND1, si-CCND1) could attenuate the inhibitory effects of ADAMTS9-AS1 overexpression on PCa cell growth.
CONCLUSIONS
Collectively, our results indicate that ADAMTS9-AS1 suppresses PCa progression by regulating the miR-142-5p/CCND1 axis, which provides a new mechanism for the progression of PCa.
背景
新出现的证据表明长非编码 RNA 在癌症发展中具有重要作用，包括前列腺癌 (PCa)。生物信息学分析表明 ADAMTS9-AS1 可能在癌症进展中发挥作用。
方法
采用实时定量聚合酶链反应检测信使 RNA 水平的 ADAMTS9-AS1 表达水平。进行体外功能分析以研究不同条件下细胞行为状态。此外，进行挽救实验以探讨 ADAMTS9-AS1 在 PCa 进展中的潜在机制。
结果
ADAMTS9-AS1 在 PCa 中表达下调。强制表达 ADAMTS9-AS1 通过诱导细胞凋亡来抑制 PCa 细胞增殖。重要的是，miR-142-5p（miR-142-5p）模拟物和靶向细胞周期蛋白 D1 (CCND1, si-CCND1) 的小干扰 RNA 可以减弱 ADAMTS9-AS1 过表达对 PCa 细胞生长的抑制作用。
结论
总之，我们的研究结果表明，ADAMTS9-AS1 通过调节 miR-142-5p/CCND1 轴抑制 PCa 进展，为 PCa 进展提供了新的机制。
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