海藻酸诱发癫痫发作期间海马苔藓纤维锌染色的丧失：一项组织荧光研究。

Loss of zinc staining from hippocampal mossy fibers during kainic acid induced seizures: a histofluorescence study.

作者信息

Frederickson C J, Hernandez M D, Goik S A, Morton J D, McGinty J F

机构信息

Laboratory for Neurobiology, University of Texas at Dallas, Richardson 75080.

出版信息

Brain Res. 1988 Apr 19;446(2):383-6. doi: 10.1016/0006-8993(88)90899-2.

DOI:10.1016/0006-8993(88)90899-2

PMID:3370496

Abstract

A quinoline fluorescence method for staining zinc in axonal boutons was used to study the effects of kainic acid (KA) induced seizures upon zinc in the boutons of hippocampal mossy fibers. Compared to untreated rats, rats given KA (10-12 mg/kg) and undergoing sustained seizures showed a marked loss of zinc fluorescence in the mossy fiber regions. The reduced fluorescence was detectable within 3 h of KA administration, was most pronounced at 12-24 h, and was still noticeable up to 48 h after KA. The findings suggest that zinc is released rapidly from mossy fiber boutons during seizures.

摘要

采用喹啉荧光法对轴突终扣中的锌进行染色，以研究海藻酸（KA）诱导的癫痫发作对海马苔藓纤维终扣中锌的影响。与未处理的大鼠相比，给予KA（10 - 12 mg/kg）并经历持续癫痫发作的大鼠，其苔藓纤维区域的锌荧光明显丧失。荧光减少在给予KA后3小时内即可检测到，在12 - 24小时最为明显，直到KA给药后48小时仍很明显。这些发现表明，癫痫发作期间锌从苔藓纤维终扣中迅速释放。