Cronin J, Dudek F E
Department of Psychology, Tulane University, New Orleans, LA 70118.
Brain Res. 1988 Nov 22;474(1):181-4. doi: 10.1016/0006-8993(88)90681-6.
Kainic acid (KA) injections destroy hippocampal pyramidal cells, induce recurrent collateral sprouting of the hilar mossy fibers (MFs), and lead to chronic seizures. In the present study, rats were injected systemically with KA (14 mg/kg) to determine whether the subsequent occurrence of seizures was correlated with MF sprouting, as indicated by Timm's staining of proximal dendrites. At 4 weeks, 56% of the KA-treated rats had MF sprouting in the temporal (ventral) hippocampus, and 52% had shown seizures between 10 and 28 days. Both seizures and sprouting were seen in 32% of the treated animals. While Timm's staining of MFs in the inner molecular layer was not directly correlated with seizure scores, animals which exhibited chronic seizures had significantly more sprouting than animals which did not have seizures.
海人酸（KA）注射会破坏海马锥体细胞，诱导门区苔藓纤维（MFs）反复侧支发芽，并导致慢性癫痫发作。在本研究中，对大鼠进行全身注射KA（14毫克/千克），以确定随后癫痫发作的发生是否与MF发芽相关，近端树突的Timm染色可表明这一点。4周时，56%接受KA治疗的大鼠在颞叶（腹侧）海马中有MF发芽，52%在10至28天之间出现了癫痫发作。32%的治疗动物同时出现了癫痫发作和发芽。虽然内分子层MFs的Timm染色与癫痫评分没有直接相关性，但出现慢性癫痫发作的动物比未出现癫痫发作的动物有明显更多的发芽。
