Department of Pharmaceutical Sciences, IFTM University, Moradabad, Uttar Pradesh, India; IPS College of Pharmacy, Gwalior, Madhya Pradesh, India.
IPS College of Pharmacy, Gwalior, Madhya Pradesh, India.
J Ethnopharmacol. 2021 Jun 12;273:114001. doi: 10.1016/j.jep.2021.114001. Epub 2021 Mar 9.
Stem bark of Anogeissus latifolia Roxb. (Family: Combretaceae) is used traditionally and ethnomedicinally for correction of kidney disorders.
The present study demonstrates the nephroprotective potential of stem bark of A. latifolia Roxb.
The HPTLC fingerprint and HPLC analysis were carried out to standardize the ethanolic extract of stem bark of A. latifolia (ALEE) using ellagic acid as a marker. Nephrotoxicity was induced in adult Wistar albino rats by gentamicin (100 mg/kg, intraperitoneally for 8 days) and they were treated with ALEE (100, 200 and 400 mg/kg, orally for 8 days), ellagic acid (10 mg/kg, orally for 8 days) and cystone syrup (5 ml/kg, orally), a standard reference a polyherbal formulation. Urine volume, serum and urine levels of creatinine, urea and uric acid, oxidative stress parameters (lipid peroxidation, catalase, superoxide dismutase and reduced glutathione), inflammatory markers (TNF-α and IL-6) and kidney weight along with its histological changes were studied in experimental animals.
HPTLC, HPLC and LC-MS analysis of ALEE revealed the presence of ellagic acid and other various phytoconstituents. Administration of gentamicin caused significant increase in urine output and kidney weight, elevated biochemical, inflammatory and oxidative stress parameters as well as caused histological damage in the kidney tissue. These parameters were attenuated by the concurrent treatment with ALEE and ellagic acid. The effects were comparable to cystone.
Present investigations concluded that ALEE exhibited nephroprotective potential and validated the traditional use of stem bark of A. latifolia in kidney disorders. The nephroprotective effect may be attributed to the antioxidant and anti-inflammatory phytoconstituents in ALEE.
Anogeissus latifolia Roxb.（山龙眼科）的茎皮在传统医学和民族医学中被用于纠正肾脏疾病。
本研究旨在证明 Anogeissus latifolia Roxb.茎皮的肾脏保护潜力。
使用鞣花酸作为标记物，通过 HPTLC 指纹图谱和 HPLC 分析对 A. latifolia 的乙醇提取物（ALEE）进行标准化。通过庆大霉素（100mg/kg，腹腔内注射 8 天）诱导成年 Wistar 白化大鼠产生肾毒性，并用 ALEE（100、200 和 400mg/kg，口服 8 天）、鞣花酸（10mg/kg，口服 8 天）和胱氨酸糖浆（5ml/kg，口服）进行治疗，胱氨酸糖浆是一种标准的多草药配方。研究了实验动物的尿液量、血清和尿液中肌酐、尿素和尿酸水平、氧化应激参数（脂质过氧化、过氧化氢酶、超氧化物歧化酶和还原型谷胱甘肽）、炎症标志物（TNF-α 和 IL-6）以及肾脏重量及其组织学变化。
ALEE 的 HPTLC、HPLC 和 LC-MS 分析显示存在鞣花酸和其他各种植物成分。庆大霉素给药导致尿液排出量和肾脏重量显著增加，生化、炎症和氧化应激参数升高，并导致肾脏组织发生组织学损伤。这些参数通过与 ALEE 和鞣花酸的同时治疗得到缓解。效果与胱氨酸相当。
目前的研究得出结论，ALEE 表现出肾脏保护潜力，验证了 Anogeissus latifolia 茎皮在肾脏疾病中的传统用途。这种肾脏保护作用可能归因于 ALEE 中的抗氧化和抗炎植物成分。
