Department of Chemistry, University of São Paulo, Av. Bandeirantes, 3900, CEP 14040-901 Ribeirão Preto, Brazil.
Department of Chemistry, University of São Paulo, Av. Bandeirantes, 3900, CEP 14040-901 Ribeirão Preto, Brazil.
Bioresour Technol. 2021 Jun;329:124929. doi: 10.1016/j.biortech.2021.124929. Epub 2021 Mar 4.
Sugarcane straw (SCS) was pretreated with dilute sulfuric acid assisted by microwave to magnify fermentable sugars and to minimize the concentration of inhibitors in the hydrolysates. The optimum conditions for maximum recovery of sugars were 162 °C and 0.6% (w/v) HSO. The low level of inhibitors, such as acetate (2.9 g/L) and total phenolics (1.4 g/L), in the SCS slurry from the pretreatment stage allowed the enzymatic hydrolysis and fermentation steps to occur without detoxification. Besides consuming the total sugar content (31.0 g/L), Clostridium beijerinckii Br21 was able to use acetate from the SCS hydrolysate, to give butyric acid at high conversion factor (0.49 g of butyric acid /g of sugar). The optimized pretreatment conditions spared acid, time, and the detoxification stage, making bio-butyric acid production from SCS extremely attractive.
甘蔗渣（SCS）经稀硫酸预处理并辅以微波处理，以放大可发酵糖并最小化水解物中抑制剂的浓度。糖最大回收率的最佳条件为 162°C 和 0.6%（w/v）HSO。预处理阶段的低水平抑制剂，如乙酸（2.9 g/L）和总酚类（1.4 g/L），使 SCS 浆中的酶水解和发酵步骤能够在无需解毒的情况下进行。除了消耗总糖含量（31.0 g/L）外，拜氏梭菌 Br21 还能够利用 SCS 水解物中的乙酸，以高转化率（0.49 g 丁酸/g 糖）产生丁酸。优化的预处理条件节省了酸、时间和解毒阶段，使 SCS 生产生物丁酸极具吸引力。
