Department of Mechanical and Aerospace Engineering, University of California San Diego Jacobs School of Engineering, 9500 Gilman Drive #0411, La Jolla, CA, 92093, USA.
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, 94720, USA.
Curr Opin Cell Biol. 2021 Aug;71:38-45. doi: 10.1016/j.ceb.2021.01.011. Epub 2021 Mar 8.
The budding of membranes and curvature generation is common to many forms of trafficking in cells. Clathrin-mediated endocytosis, as a prototypical example of trafficking, has been studied in great detail using a variety of experimental systems and methods. Recently, advances in experimental methods have led to great strides in insights on the molecular mechanisms and the spatiotemporal dynamics of the protein machinery associated with membrane curvature generation. These advances have been ably supported by computational models, which have given us insights into the underlying mechanical principles of clathrin-mediated endocytosis. On the other hand, targeted experimental perturbation of membranes has lagged behind that of proteins in cells. In this area, modeling is especially critical to interpret experimental measurements in a mechanistic context. Here, we discuss the contributions made by these models to our understanding of endocytosis and identify opportunities to strengthen the connections between models and experiments.
膜的出芽和曲率生成在细胞内的许多运输形式中都很常见。网格蛋白介导的内吞作用作为运输的典型范例，已经使用各种实验系统和方法进行了详细研究。最近，实验方法的进步使得我们对与膜曲率生成相关的蛋白质机制的分子机制和时空动力学有了很大的了解。这些进展得到了计算模型的有力支持，这些模型使我们深入了解网格蛋白介导的内吞作用的潜在机械原理。另一方面，与细胞内的蛋白质相比，针对膜的靶向实验干扰落后了。在这一领域，建模对于在机械背景下解释实验测量结果尤其重要。在这里，我们讨论了这些模型对我们对内吞作用的理解的贡献，并确定了加强模型与实验之间联系的机会。
