Du Fan, Huang Rongfeng, Lin Dan, Wang Yuying, Yang Xiaohuang, Huang Xiaoyun, Zheng Biyun, Chen Zhixin, Huang Yuehong, Wang Xiaozhong, Chen Fenglin
Department of Gastroenterology, Fujian Medical University Union Hospital, Fuzhou, China.
Fujian Medical University Cancer Center, Fujian Medical University, Fuzhou, China.
Front Microbiol. 2021 Feb 23;12:611323. doi: 10.3389/fmicb.2021.611323. eCollection 2021.
Resveratrol (RSV) is a potential alternative therapy for non-alcoholic fatty liver disease (NAFLD) that has been evaluated in many clinical trials, but the mechanisms of RSV action have not been fully elucidated. Recent studies suggested that the gut microbiota is an important RSV target; therefore, we speculated that the gut microbiota might mediate the beneficial effects of RSV in NAFLD. To verify this hypothesis, we established a high-fat diet (HFD)-induced NAFLD mouse model, which was subjected to RSV gavage to evaluate the therapeutic effects. We observed that RSV reduced liver steatosis and insulin resistance in NAFLD. RSV significantly changed the diversity and composition of the gut microbiota according to 16S rRNA sequencing. Gut microbiota gene function prediction showed that the enrichment of pathways related to lipid and glucose metabolism decreased after RSV treatment. Furthermore, correlation analysis indicated that the improvements in NAFLD metabolic indicators were closely related to the altered gut microbiota. We further fermented RSV with the gut microbiota to verify that RSV directly affected the gut microbiota. Our data suggested that the gut microbiota might be an important target through which RSV exerts its anti-NAFLD effect.
白藜芦醇（RSV）是一种用于非酒精性脂肪性肝病（NAFLD）的潜在替代疗法，已在许多临床试验中进行了评估，但RSV的作用机制尚未完全阐明。最近的研究表明，肠道微生物群是RSV的一个重要靶点；因此，我们推测肠道微生物群可能介导了RSV对NAFLD的有益作用。为了验证这一假设，我们建立了高脂饮食（HFD）诱导的NAFLD小鼠模型，并对其进行RSV灌胃以评估治疗效果。我们观察到RSV减轻了NAFLD中的肝脏脂肪变性和胰岛素抵抗。根据16S rRNA测序，RSV显著改变了肠道微生物群的多样性和组成。肠道微生物群基因功能预测表明，RSV治疗后与脂质和葡萄糖代谢相关的途径富集减少。此外，相关性分析表明，NAFLD代谢指标的改善与肠道微生物群的改变密切相关。我们进一步用肠道微生物群发酵RSV，以验证RSV直接影响肠道微生物群。我们的数据表明，肠道微生物群可能是RSV发挥其抗NAFLD作用的一个重要靶点。
