Hao Hu, Gu Xia, Cai Yao, Xiong Hui, Huang Liping, Shen Wei, Ma Fei, Xiao Xin, Li Sitao
Department of Neonatology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Obstetrics and Gynecology, Southern Medical University, Nanfang Hospital, Guangzhou, China.
Transl Pediatr. 2021 Feb;10(2):296-305. doi: 10.21037/tp-20-211.
Pregnancy-induced hypertension (PIH) is associated with an increased number of neonatal complications, but its impact on neonatal metabolism remains unclear. This study aimed to investigate the differential metabolomics of infants born to mothers with and without PIH.
Blood samples of a total of 115 infants born to mothers with (n=56) and without (n=59) PIH were collected and assigned to two groups, respectively, from the neonatal department of Sixth Affiliated Hospital of Sun Yat-Sen University. A tandem mass spectrometer was used to generate metabolic profiling of amino acid, free carnitine, and acyl-carnitines. The resulting data were analyzed using orthogonal partial least squares discriminant analysis based on the difference between infants born to mothers with or without PIH.
A significant relationship was observed between the two groups (with and without PIH) in the metabolic fingerprint. According to the pattern recognition analysis combined with variance importance, 25 metabolites with high importance were found. The top ten substances were selected for analysis. Compared with infants born to mothers without PIH, glycine levels increased, and C14DC, C22, C4DC, C5:1, C6DC, C5-OH, proline, C14-OH, and C20 decreased in infants born to mothers with PIH.
Using liquid chromatography (LC)-MS/MS metabolomics, a significant relationship was detected between neonatal metabolism and maternal hypertension. It is important to correct the subsequent infantile metabolic disorder by balancing the biomarker metabolites and suppling adequate nutrition to improve the health and growth of newborns of PIH mothers.
妊娠期高血压（PIH）与新生儿并发症数量增加有关，但其对新生儿代谢的影响尚不清楚。本研究旨在调查患PIH和未患PIH母亲所生婴儿的差异代谢组学。
从中山大学附属第六医院新生儿科收集了总共115名母亲患PIH（n = 56）和未患PIH（n = 59）的婴儿的血样，并分别分为两组。使用串联质谱仪生成氨基酸、游离肉碱和酰基肉碱的代谢谱。基于患PIH和未患PIH母亲所生婴儿之间的差异，使用正交偏最小二乘法判别分析对所得数据进行分析。
两组（患PIH和未患PIH）在代谢指纹图谱上存在显著关系。通过模式识别分析结合变量重要性，发现了25种具有高度重要性的代谢物。选择前十种物质进行分析。与未患PIH母亲所生婴儿相比，患PIH母亲所生婴儿的甘氨酸水平升高，而C14DC、C22、C4DC、C5:1、C6DC、C5-OH、脯氨酸、C14-OH和C20降低。
使用液相色谱（LC）-MS/MS代谢组学，检测到新生儿代谢与母亲高血压之间存在显著关系。通过平衡生物标志物代谢物并提供充足营养来纠正随后的婴儿代谢紊乱，对于改善PIH母亲所生新生儿的健康和生长非常重要。
