Department of Pediatrics, The Sixth Affiliated Hospital, Sun Yat-sen University, 510655, Guangzhou, China.
Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, 510530, Guangzhou, China.
Eur J Pediatr. 2023 Nov;182(11):5015-5024. doi: 10.1007/s00431-023-05171-1. Epub 2023 Aug 30.
This study aimed to compare the blood metabolic status of neonates with idiopathic polyhydramnios (IPH) and those with normal amniotic fluid, and to explore the relationship between IPH and fetal health. Blood metabolites of 32 patients with IPH and 32 normal controls admitted to the Sixth Affiliated Hospital of Sun Yat-sen University between January 2017 and December 2022 were analyzed using liquid chromatography-mass spectrometry (LC-MS/MS). Orthogonal partial least squares discriminant analysis (OPLS-DA) and metabolite enrichment analyses were performed to identify the differential metabolites and metabolic pathways. There was a significant difference in the blood metabolism between newborns with IPH and those with normal amniotic fluid. Six discriminant metabolites were identified: glutamate, serine, asparagine, aspartic acid, homocysteine, and phenylalanine. Differential metabolites were mainly enriched in two pathways: aminoacyl-tRNA biosynthesis, and alanine, aspartate, and glutamate metabolism.
This study is the first to investigate metabolomic profiles in newborns with IPH and examine the correlation between IPH and fetal health. Differential metabolites and pathways may affect amino acid synthesis and the nervous system. Continuous attention to the development of the nervous system in children with IPH is necessary.
• There is an increased risk of adverse pregnancy outcomes with IPH, such as perinatal death, neonatal asphyxia, neonatal intensive care admission, cesarean section rates, and postpartum hemorrhage. • Children with a history of IPH have a higher proportion of defects than the general population, particularly central nervous system problems, neuromuscular disorders, and other malformations.
• In neonates with IPH, six differential metabolites were identified with significant differences and good AUC values using LC-MS/MS analysis: glutamic acid, serine, asparagine, aspartic acid, homocysteine, and phenylalanine, which were mainly enriched in two metabolic pathways: aminoacyl-tRNA biosynthesis and alanine, aspartate, and glutamate metabolism. • These differential metabolites and pathways may affect amino acid synthesis and development of the nervous system in neonates with IPH.
本研究旨在比较特发性羊水过多（IPH）新生儿与羊水正常新生儿的血液代谢状态，并探讨 IPH 与胎儿健康的关系。方法：采用液相色谱-质谱联用（LC-MS/MS）技术分析 2017 年 1 月至 2022 年 12 月中山大学附属第六医院收治的 32 例 IPH 患者和 32 例正常对照者的血液代谢产物。采用正交偏最小二乘法判别分析（OPLS-DA）和代谢物富集分析鉴定差异代谢物和代谢途径。结果：IPH 新生儿与羊水正常新生儿的血液代谢存在显著差异。共鉴定出 6 种差异代谢物：谷氨酸、丝氨酸、天冬酰胺、天冬氨酸、同型半胱氨酸和苯丙氨酸。差异代谢物主要富集在两个途径中：氨酰-tRNA 生物合成和丙氨酸、天冬氨酸和谷氨酸代谢。结论：本研究首次探讨了 IPH 新生儿的代谢组学特征，并研究了 IPH 与胎儿健康的相关性。差异代谢物和途径可能影响氨基酸合成和神经系统发育。应对 IPH 患儿的神经系统发育进行持续关注。
