Farzanehpour Mahdieh, Faghihloo Ebrahim, Salimi Vahid, Jalilvand Somayeh, Akhavan Setareh, Muhammadnejad Ahad, Emami Razavi Amir Nader, Kakavandi Ehsan, Mokhtari Azad Talat
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2020 Nov;49(11):2179-2188. doi: 10.18502/ijph.v49i11.4736.
Molecular profiling techniques are the rapid detection of biomarkers in the human papillomavirus (HPV) infected cells. We aimed to measure the expression level of three cell factors including Snail1, ZEB-1, and E-cadherin in cervical cancer (CC), precancerous and healthy samples, simultaneously, to find potential biomarkers.
The expression level of the mentioned cell factors were investigated in 72 CC patients, precancerous patients, and healthy controls by using Real-Time PCR.
The results demonstrated a significant reduction in the expression level of E-cadherin in cancer and precancerous cases than that in healthy cases; whereas the expression level of ZEB-1 and Snail1 were upregulated in cancer and precancerous samples. The receiver operating characteristic (ROC) analyses shows the highest AUC value emerged for Snail1: 1(95% CI: 1-1) in comparing CC and healthy groups with a sensitivity of 100.0 % and specificity of 100.0%.
The molecular biomarker Snail1 may be helpful to early diagnosis and prognosis of CC in the HPV-infected human populations. Considering the increased expression level of Snail1 in cancer and precancerous tissue compared to healthy tissue as well as the area under the ROC curve, Snail1 can be used for early detection of CC.
分子谱分析技术可快速检测人乳头瘤病毒（HPV）感染细胞中的生物标志物。我们旨在同时测量宫颈癌（CC）、癌前病变和健康样本中三种细胞因子Snail1、ZEB-1和E-钙黏蛋白的表达水平，以寻找潜在的生物标志物。
通过实时聚合酶链反应对72例CC患者、癌前病变患者和健康对照者中上述细胞因子的表达水平进行研究。
结果显示，与健康样本相比，癌症和癌前病变病例中E-钙黏蛋白的表达水平显著降低；而ZEB-1和Snail1的表达水平在癌症和癌前病变样本中上调。受试者工作特征（ROC）分析显示，在比较CC组和健康组时，Snail1的曲线下面积（AUC）值最高：1（95%置信区间：1-1），灵敏度为100.0%，特异性为100.0%。
分子生物标志物Snail1可能有助于HPV感染人群中CC的早期诊断和预后评估。鉴于与健康组织相比，Snail1在癌症和癌前病变组织中的表达水平升高以及ROC曲线下面积，Snail1可用于CC的早期检测。
