血清和组织中的 microRNAs：宫颈癌诊断的潜在生物标志物。
Serum and tissue miRNAs: potential biomarkers for the diagnosis of cervical cancer.
机构信息
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 1471613151, Iran.
Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
出版信息
Virol J. 2019 Oct 7;16(1):116. doi: 10.1186/s12985-019-1220-y.
BACKGROUND
Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects.
METHODS
The expression levels of tissue and serum miRNAs, including miR-9, miR-192 and miR-205, were investigated in 36 normal, 18 precancer, and 18 cervical cancer samples using real-time polymerase chain reaction.
RESULTS
The results showed the higher significant expressions of miR-9, miR-192 and miR-205 in the tissue of cancer samples than those in the normal samples. Moreover, the miR-192 and miR-205 expression were significantly increased in the cancer group in comparison with the precancer group. Examination of serum samples revealed the increase in the expression level in the cancer groups than in the normal samples, for miR-9, miR-192 and miR-205 and the expressions of miR-9, miR-192 and miR-205 were significantly up-regulated in the precancer group in comparison with the normal group. Also the expression of miR-205 was remarkably increased in the cancer group in comparison with the precancer group. The receiver operating characteristic (ROC) analyses showed the highest area under the curve value for miR-192.
CONCLUSIONS
Given the increased expression level of miR-192 in cancer and in precancerous tissue and serum compared with the normal tissue and serum validated by analysing the ROC curve, miR-192 can be used as potential biomarker for the early detection of cervical cancer.
背景
寻找用于宫颈癌早期检测的新生物标志物是该领域的基本要求。在这项研究中，我们旨在评估与正常受试者相比，宫颈癌患者宫颈癌进展中潜在生物标志物的表达水平。
方法
使用实时聚合酶链反应检测 36 例正常、18 例癌前病变和 18 例宫颈癌样本中的组织和血清微小 RNA（miRNA），包括 miR-9、miR-192 和 miR-205 的表达水平。
结果
结果表明，癌症样本中的 miR-9、miR-192 和 miR-205 的表达明显高于正常样本。此外，miR-192 和 miR-205 在癌症组中的表达与癌前病变组相比显著增加。检测血清样本显示，miR-9、miR-192 和 miR-205 在癌症组中的表达水平高于正常组，miR-9、miR-192 和 miR-205 在癌前病变组中的表达水平明显高于正常组。此外，miR-205 在癌症组中的表达与癌前病变组相比显著增加。受试者工作特征（ROC）分析显示 miR-192 的曲线下面积值最高。
结论
鉴于 miR-192 在癌症和癌前组织以及血清中的表达水平高于正常组织和血清，ROC 曲线分析验证了 miR-192 可以作为宫颈癌早期检测的潜在生物标志物。
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